响应面法优化竹笋壳中棕色素提取工艺

Optimization of Brown Pigment Extraction from Bamboo Shoot Shell by Response Surface Methodology

研究了超声辅助的方法从竹笋壳中提取棕色素的工艺。首先以NaOH溶液质量浓度、料液比、提取温度和提取时间为影响因素进行了单因素试验,在此基础上,运用Box-Behnken中心组合试验和响应面法考察了NaOH溶液质量浓度、提取温度、提取时间3个因素对棕色素提取率的影响,并优化了提取工艺。结果表明,最佳工艺条件为:NaOH溶液质量浓度6.6 g/L,提取温度78℃,提取时间1.8h。在此条件下,棕色素提取率的预测值为0.317 g/g,验证试验值的棕色素提取率为0.3004g/g,说明响应面法优化竹笋壳中棕色素的提取工艺可行。

Brown pigment which extracted from bamboo shoot shell using ultrasonic was studied. Based on the single factor tests of the NaOH solution mass concentration, liquid-solid ratio, extraction time and extraction temperature, the optimum extraction process was evaluated by Box-Behnken central composite experimental design and response surface methodology analysis. The optimum conditions were as follows： the mass concentration of sodium hydroxide solution was 6.6 g/L, the extraction temperature was 78 ℃, and the extraction time was 1.8 h. With these optimized conditions, the experimental value of brown pigment extraction yield was 0.3004 g/g, in good agreement with the predictive value 0.317 g/g. Therefore, the brown pigment extraction process from bamboo shoot shell which optimized by response surface methodology was very reliable.