川芎、赤芍多糖活性分析及其比较

Analyze and Compare Activities of Polysaccharide form Rhizoma Chuanxiong and Radix Paeoniae Rubra

目的:探讨2种中药多糖的生物活性及其活性上的差异。方法:采用水提醇沉法提取多糖并进行含量测定,比浊法测定抑菌活性,采用三个体外抗氧化指标测定抗氧化活性,采用MTT比色法测定对细胞生长的影响。结果:2种多糖在抗菌、抗氧化以及促进细胞生长方面都表现一定活性,且存在明显的活性差异。抗氧化以及抗菌活性上赤芍多糖强于川芎多糖;促细胞生长活性上川芎多糖强于赤芍多糖。结论:两者存在活性上的差异,表现出一定的互补效应。这种活性差异,可以在复方中组合应用。

Objective ：To explore the difference of biological activities between two Chinese Herbs polysaccharides. Methods：Extracted two kinds of Chinese Herbs Polysaccharides by hot water, and determined the content of Polysaccharides. Determined the antibacterial activity with turbidimetry. Explored antioxidant activity using an in vitro antioxidant analysis, probed into the effects on cell growth with MTr colorimetric analysis. Results：The two polysaccharides both showed activities on the antibacterial, anti-oxidation and promotion of cell growth but different in degree. The Radix Paeoniae Rubra polysaccharide was stronger than Rhizoma Chuanxiong in antioxidant and antimicrobial activities,and weaker in promotion of cell growth activity. Conclusion：Activity differences exist between the two polysaccharides and the activities show some complementary effects. The differences provide important value in research on polysaccharide compound.