响应面法优化超临界流体萃取枇杷叶中熊果酸的研究

Optimization of Supercritical Fluid Extraction of Ursolic Acid from Follum Eriobotryae by Response Surface Methodology

目的:研究超临界二氧化碳萃取枇杷叶中熊果酸的工艺。方法:通过单因素试验和响应面优化,用高效液相测定萃取物中熊果酸含量;建立枇杷叶中熊果酸超临界萃取的数学模型,确定最佳萃取工艺条件。结果:熊果酸超临界萃取的最佳工艺条件为:萃取温度61.6℃,萃取压力25.8 MPa,动态萃取时间40 min,在此条件下熊果酸的理论提取率为3.96 mg/g。结论:优化得到的该萃取工艺可靠。

Objective ：To optimize the conditions for extracting ursolic acid from Follum eriobotryae with supereritical fluid extra- tion （SFE） . Methods：The contents of ursolie acid in the extracts were determined by HPLC. Based on single factor experiment and response surface methodology, a mathematical model for SFE of ursolic acid was built. Results ： The result showed that the optimum condition paremeters were as follows： temperature 61.6~C, extraction pressure 25.8 MPa, dynamic extraction time 40 min. Under these conditions, theoretical extraction rate of ursolie acid was 3.96 mg/g. Conclusion：The eptimal process is reliable.