诱导型环氧合酶高表达增加兔主动脉粥样硬化斑块易损性

Overexpression of COX-2 enhances rabbit atherosclerotic plaque vulnerability

目的:探讨诱导型环氧合酶(COX-2)在兔主动脉粥样硬化(atherosclerosis,AS)组织中的表达及其与斑块易损性之间的关系。方法:以高脂饲料喂养建立兔主AS模型,并与正常饲料组对照。喂养12周后,取兔主动脉标本,行HE及免疫组化染色,观察COX-2、基质金属蛋白酶-2(MMP-2)、Ⅰ型和Ⅲ型胶原在AS斑块组织中的表达。结果:COX-2仅见于AS斑块,正常动脉壁无表达。AS组COX-2免疫组化染色强度均显著高于正常对照组(P<0.05)。AS组Ⅰ、Ⅲ型胶原和MMP-2免疫组化染色强度均显著高于正常对照组(P<0.05),Ⅰ/Ⅲ型胶原的比例增加。结论:COX-2在兔主动脉AS斑块中的高表达可能增加斑块的易损性。

AIM： To evaluate the expression of COX-2 in rabbit atherosclerotic plaque and the relationship between COX-2 and plaque vulnerability.METHODS： The rabbit aortic atherosclerotic model was developed by high cholesterol feeding.Rabbit aorta specimens were measured by HE staining and immunohistochemical staining of COX-2,MMP-2 and I/III collagen.RESULTS： COX-2 was found in atherosclerotic plaques but not in healthy or nonatherosclerotic vascular walls.COX-2,MMP-2 and I/III collagen was induced in atherosclerotic plaque lesions,implicating a relationship between COX-2 and plaque vulnerability.CONCLUSION： Overexpression of COX-2 enhances rabbit atherosclerotic plaque vulnerability.