水飞蓟素对非酒精性脂肪性肝炎大鼠小肠组织抗氧化作用的实验研究

Anti-oxidant effects of silymarin on intestinal tissues in rats with non-alcoholic steatohepatitis

目的探讨水飞蓟素对NASH大鼠小肠组织氧化应激损伤的影响。方法 50只Wistar大鼠被随机分为对照组、模型组和水飞蓟素干预组;对照组(10只)用普通饲料喂养,模型组(20只)通过高脂饮食建立NASH模型,水飞蓟素组(20只)给予高脂饮食加水飞蓟素灌胃,12周后评估。结果模型组较正常对照组大鼠血清TG、TC、ALT、AST水平明显升高(P<0.01),水飞蓟素组TG、TC、ALT、AST水平较模型组降低,差别有统计学意义(F=36.626～64.327,P<0.01);模型组大鼠较正常对照组小肠组织匀浆SOD活性明显降低(83.29±10.32U/mgprot对52.34±7.09U/mgprot),MDA含量明显升高(0.56±0.07 Nmol/mgprot对0.97±0.09 Nmol/mgprot),水飞蓟素组SOD活性较模型组有所升高,MDA水平下降,差异有统计学意义(F=23.488和10.609,P<0.05);内毒素水平为模型组(0.316±0.020EU/ml)>水飞蓟素组(0.279±0.022EU/ml)>正常对照组(0.234±0.021EU/ml)s;IgA水平为模型组<水飞蓟素组<正常对照组。结论抗氧化剂水飞蓟素不仅能提高肝脏本身的抗氧化能力,亦可以通过提高肠道抗氧化能力进而加强肠道的屏障作用。

Objective To explore the anti-oxidant effects of silymarin on intestinal tissues in rats with non-alcoholic steatohepatitis.Methods Fifty Wistar rats were randomly divided into control,model and silymarin groups.The rats in control group were fed with normal diet.The rats in model group were fed with fat rich diet and saline by gastric lavage method to establish the model of NASH.The rats in silymarin group were fed with fat rich diet and silymarin by gastric lavage method.All rats in each group were observed for 12 weeks.Results The serum levels of TC,TG,ALT and AST were higher in model group than those in control group.The serum levels of TC,TG,ALT and AST were lower in silymarin group than those in model group（F=36.626～64.327,P0.01）;The activitives of SOD were lower in model group than in control group（83.29±10.32 U/mgprot vs.52.34±7.09 U/mgprot）.The activitives of SOD in silymarin group were higher than those in model group;The changes of MDA were on the contrary（0.56±0.07 Nmol/mgprot vs.0.97±0.09 Nmol/mgprot,F=23.488 and 10.609,respectively,P0.05）;The levels of ET in model group（0.316±0.020EU/ml）were higher than in silymarin grou p（0.279±0.022EU/ml）.Conclusion Silymarin,an anti-oxidant drug,can protect the hepatocytes and the intestines in rats with NASH.