苍术中β-桉叶油醇和茅术醇的分离制备研究

Research on the Separation and Preparation of β-eudesmol and Hinesol from Rhizoma Atractylodis

目的:研究苍术挥发油中两个主要成分β-桉叶油醇和茅术醇的分离方法,为研究麸炒对苍术挥发油的影响提供对照品。方法:采用硅胶、硝酸银硅胶柱色谱、Sephadex LH-20凝胶柱色谱进行分离纯化,采用核磁共振(1 H-NMR、13 C-NMR)对其进行结构鉴定。结果:从苍术药材中分离得到β-桉叶油醇、茅术醇作为对照品。结论:对于β-桉叶油醇、茅术醇所建立的分离、纯化方法简便可行。

Objective：To investigate the separation methods of two main ingredients β-eudesmol and hinesol from volatile oil in Rhizoma atractylodis,to provide chemical references for investigating the effect of processing on volatile oil in Rhizoma atractylodis.Method：Isolation and purification were carried out on the chromatography of silica gel、AgNO3 impregnated silica gel and Sephadex LH-20;The structures were determined by NMR（1H-NMR、13C-NMR）.Result：β-Eudesmol and Hinesol were screened out as the chemical references of Rhizoma atractylodis.Conclusion：The methods of isolation and puirfication for β-eudesmol and hinesol reported in this paper are simple and feasible.