庆大霉素-壳聚糖纳米涂层钛板的制备及系列研究

Preparation and series studies of the gentamicin sulfate/chitosan nanospheres loaded plates

目的:制备负载庆大霉素-壳聚糖纳米粒的涂层钛板并对其表面形态、体外释药行为及抗金黄色葡萄球菌的性能进行评价。方法:采用溶剂铸膜(solvent casting)法制备庆大霉素-壳聚糖纳米涂层钛板,扫描电镜观察其表面形态,磷酸盐缓冲液中研究其体外释药行为,将庆大霉素-壳聚糖纳米涂层钛板置于均匀涂有1×108 CFU/ml金黄色葡萄球菌的Mueller-Hinton(MH)平板上连续培养直至抑菌环消失,以抑菌环直径为主要观察指标评价其在体外对金黄色葡萄球菌的抑制作用。结果:扫描电镜示庆大霉素-壳聚糖纳米粒较均匀地分布于钛板表面;体外释药行为显示庆大霉素-壳聚糖纳米涂层钛板中庆大霉素的释放可达到14 d,维持在52%左右;体外抑制金黄色葡萄球菌作用可维持9 d,抑菌环最大直径为(3.82±0.03)cm。结论:制备的庆大霉素-壳聚糖纳米涂层钛板表面形态良好,体外释药稳定,对金黄色葡萄球菌有良好的抑制作用。

Objective： To develop the gentamicin sulfate/chitosan nanospheres loaded plates and evaluate its characteristics such as surface structure and the drug release behavior of the drug in vitro and its anti-Staph aureus efficacy in vitro.Methods： The solvent casting technology was adopted to prepare the gentamicin sulfate/chitosan nanospheres loaded plates.The surface appearance of the plates was observed by SEM,the drug release behavior in vitro was studied by immerse the plates into PBS.And the gentamicin sulfate/chitosan nanospheres loaded plates were placed in the centre of the Mueller-Hinton（MH） agar which were inoculated with the Staph aureus at the concentration of 1 × 108 CFU/ml.The bacteria were cultured on MH agar for 48 h and the diameter of the inhibition zone was measured,and this procedure was repeated until there was no inhibition zone.Result： The result of the SEM shows the gentamicin sulfate/chitosan nanospheres distributed on the surface of plates uniform,the drug release behavior in vitro could continue about 14 days,and the max cumulative release of the drug was about 52%.The anti-Staph aureus efficacy in vitro could continue about 9 days and the max diameter of the inhibition zone was（3.82 ± 0.03） cm.Conclusion： The gentamicin sulfate/chitosan nanospheres loaded plates show well surface appearance and the plates have a steady drug release behavior and significant anti-Staph aureus efficacy in vitro.