高效液相色谱法测定肾炎解热片中连翘苷的含量

Determination of Forsythia in Shenyan Jiere Tablets by HPLC

建立了肾炎解热片中连翘苷含量的测定方法。采用高效液相色谱法测定连翘苷含量。以Waters SunFire C18(4.6mm×200mm)为色谱柱,以乙腈+水(33+67)为流动相,流速1.0ml.min-1,检测波长为277nm。结果:连翘苷对照品进样量在0.025～0.25μg范围内线性关系良好。方法的回收率为98.2%(n=5),RSD为1.5%。该方法快捷,准确,稳定性、重复性好。可用于肾炎解热片的质量控制。

To establish a method for quality control of Shenyan Jiere Tablets. HPLC method was developed to quantitative determination. Waters SunFire C18 column （4.6mm × 200mm） was used with acetonitrilewater （33：67） as the mobile phase. And the detection wavelength was 277 nm. Results： The calibration curves of forsythia showed that the linearity was completely acceptable between 0. 025- 0.251μg. The average recovery of forsythia was 98.2% with RSD of 1.5% （ n = 5 ）. The method is rapid, stable and repeatable, and can be used for the quality control of Shenyan Jiere Tablets.