哺乳牦牛同期发情/定时授精技术试验研究

Study on Estrus Synchronization and Timing Artificial Insemination Technology in Lactating Famale Yak

本研究旨在对不同繁殖体况的母牦牛在补饲的基础上,分别从营养、隔离断奶、激素选择考察影响母牦牛繁殖率及同期发情处理效果。选用青海省环湖地区480头5～10岁繁殖体况不同的母牦牛,按照试验要求将母牦牛分成不同的试验组别,采用相同的同期发情处理方案,通过不同程度的补饲,补饲组与对照组的排卵率和妊娠率对比差异显著(P<0.05);适时隔离断奶结果表明,隔离断奶组与对照组的妊娠率对比差异显著(P<0.05);不同激素处理结果表明,EB组、ECP组与GnRH组的排卵率及妊娠率对比差异显著(P<0.05),EB组与ECP组的排卵率及妊娠率对比差异不显著(P>0.05)。研究表明,在传统的放牧条件下,犊牛哺乳及能量负平衡是影响牦牛进入发情季节正常发情排卵的关键影响因素,导致促黄体素分泌不足及卵泡发育受阻,通过适度的补饲及隔离断乳,进而采用同期发情/定时授精技术,能够改善母牦牛的繁殖体况和卵巢机能状态,从而提高母牦牛的排卵率和妊娠率。

This experiment was conducted to investigate different breeding body condition of the female yaks based on supplementary,study effect of nutrition,temporary weaning,hormone chosen study on conception rate and estrus synchronization of female yak.480 female yaks age from 5 years to 10 years were Selected,were treated with the estrus synchronization /timing artificial insemination in around the Qinghai yaks.According to testing requirements,female yak would be divided into different experiment groups with same estrus synchronization.And the different level supplementary,There is significant difference in ovulation ratio and pregnancy rates between the supplementary group and the control group（P〈0.05）.There is significant difference in ovulation ratio and pregnancy rates between the control group and the temporary weaning group（P〈0.05）.The results showed that there is significant difference in ovulation ratio and pregnancy rates between EB group,ECP group and GnRH group for different hormone treatment（P〈0.05）,but there is not significant difference in ovulation ratio and pregnancy rates between EB group and ECP group（P〈0.05）.The research indicates,in the traditional herding conditions,the two important factors affect female yak estrus and ovulation is calves lactation and negative energy balance which cause inadequant luteinizing-hormone secretes and follicular development limitation.Through the appropriate supplementary feeding programs and temporary weaning,then using estrus synchronization /timing artificial insemination can improve the reproductive body condition of female yaks with ovarian function status and therefore significantly improve the ovulation rate and pregnancy rate of female yak.