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SN38对Fas介导凋亡效应的影响 被引量：1

Influence of SN38 on Fas-Mediated Apoptosis Effect
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摘要 为探讨SN38与Fas介导凋亡的相互关系,并进一步探讨脂筏(富含鞘磷脂)在该过程的作用,本实验拟单独或者联合应用SN38和CH11作用于脂筏阴性和脂筏阳性的淋巴瘤细胞株WR/Fas-SM(-)细胞和WR/Fas-SMS1细胞,通过流式细胞术、DNA ladder等实验方法观察SN38能否促进Fas介导的凋亡以及对2细胞的效应差异。结果显示,SN38与CH11联合应用与单独应用SN38相比,可以诱导WR/Fas-SMS1细胞更加明显凋亡现象的发生,但对WR/Fas-SM(-)细胞凋亡的促进作用不明显,提示脂筏在SN38促进WR/Fas-SMS1细胞Fas介导凋亡过程中起到一定作用。 In order to investigate the mutual relationship between SN38 and Fas-mediated apoptosis and to further study the role of lipid raft （abundant in sphingomyelin） in the process, in the experiments SN38 and CHll were worked out to react independently or jointly on lymphoma cell strains of lipid raft positive and lipid raft negative WR/ Fas-SM（ - ） cells and WR/Fas-SMS1 cells, and observe the effect differences whether the SN38 could enhance Fasmediated apoptosis and two cells through flow cytometry, DNA ladder and other experimental methods. The results showed joint application of SN38 and CH11 to induce more noticeable apoptosis to happen in WR/Fas-SMS1 cells than in WR/Fas-SM （ - ） cells as compared with application of SN38 alone. Yet the enhancement on WR/Fas-SMS（ - ） cells apoptosis was not notlceable, prompted that lipid raft played a certain effect on the enhancement of SN38 during the process of WR/Fas-SMS1 cell Fas mediated-apoptosis.
作者 曹妍 王欢 童晓鹏 单风平 梅原久範 吕昌龙
机构地区 中国医科大学基础医学院免疫学教研室 西藏民族学院免疫学教研室 金沢医科大学血液免疫内科
出处 《微生物学杂志》 CAS CSCD 2011年第5期49-53,共5页 Journal of Microbiology
关键词 凋亡 SN38 脂筏 鞘磷脂 apoptosis SN38 lipid raft sphingomyelin
分类号 R392 [医药卫生—免疫学]
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参考文献15

  • 1Siegel R M, Frederiksen J K, Zaeharias D A, et al. Fas preassoeiation required for apoptosis signaling and dominant inhibition by pathogenic mutations[ J]. Science,2000,288 (5475) : 2354-2357.
  • 2Rothenberg M L. Irinoteean (CPT-11): recent developments and future direetions--eoloreetal cancer and beyond[ J]. Oneol- ogist,2001,6 ( 1 ) :66 -80.
  • 3Lansiaux A, Bras-Gonealves R A, Rosty C, et al. Topoisomer-ase I-DNA covalent complexes in human colorectal cancer xenografts with different p53 and microsatellite instability status: relation with their sensitivity to CTP-11 [ J ]. Anticaneer Res, 2001,21 (1A) :471-476.
  • 4Miyaji M, Jin Z X, Yamaoka S, et al. Role of membrane sphingomyelin and ceramide in platform formation for Fas-mediated apoptosis[ J]. J Exp Med,2005,202(2) :249-259.
  • 5Yamaoka S, Miyaji M, Kitano T, et al. Expression cloning of a human cDNA restoring sphingomyelin synthesis and cell growth in sphingomyelin synthase-defective lymphoid cells[ J]. J Biol Chem ,2004,279 (18) : 18688-18693.
  • 6Allen J A, Halverson-Tamboli R A and Rasenick M M. Lipid raft mierodomains and neurotransmitter signalling[ J]. Nat Rev Neuresci ,2007,8 (2) : 128-140.
  • 7Simons K, Vaz W L. Model systems, lipid rafts, and cellmembranes[ J]. Annu Rev Biophys Biomol Struct, 2004,33 : 269 -295.
  • 8Yoon I S, Chen E, Busse T, et al. Low-density lipopretein receptor-related protein promotes amyloid precursor protein trafficking to lipid rafts in the endocytic pathway [ J]. FASEB J, 2007,21 ( 11 ) :2742-2752.
  • 9Jin Z X, Huang C R, Dong L, et al. Impaired TCR signaling through dysfunction of lipid rafts in sphingomyelin synthase 1 ( SMS1 ) -knockdown T cells [ J ]. Int Immunol, 2008,20 ( 11 ) : 1427 -1437.
  • 10Auriac A, Willemetz A and Canonne-Hergaux F. Lipid raft-dependent endoeytosis : a new route for hepcidin-mediated regulation of ferroportin in macrophages[ J]. Haematologica,2010,95 (8) :1269-1277.

同被引文献19

  • 1Rothenberg M L. Irinotecan ( CPT-11 ) : recent developments and fu- ture directions--colorectal cancer and beyond[ J]. Oncologist, 2001 ; 6(1) : 66-80.
  • 2Lansiaux A, Bras-Goncalves R A, Rosty C et al. Topoisomerase I- DNA covalent complexes in human colorectal cancer xenografts with different p53 and microsatellite instability status: relation with their sensitivity to CTP-11 [J]. Anticancer Res, 2001; 21 (1A): 471- 476.
  • 3Gajate C, Mollinedo F. The antitumor ether lipid ET-18-OCH(3 ) induces apoptosis through translocation and capping of Fas/CD95 into membrane rafts in human leukemic ceils [ J ]. Blood, 2001 ; 98 (13) : 3860-3863.
  • 4Hueber A O, Bernard A M, Herincs Z et al. An essential role for membrane rafts in the initiation of Fas/CD95-triggered cell death in mouse thymocytes[J]. EMBO Rep, 2002; 3(2) : 190-196.
  • 5Miyaji M, Jin Z X, Yamaoka Set al. Role of membrane sphingomye- lin and ceramide in platform formation for Fas-mediated apoptosis [J]. J Exp Med, 2005; 202(2) : 249-259.
  • 6Yamaoka S, Miyaji M, Kitano T et al. Expression cloning of a human cDNA restoring sphingomyelin synthesis and cell growth in sphingo- myelin synthase-defective lymphoid ceils [ J ]. J Biol Chem, 2004 ; 279(18) : 18688-18693.
  • 7Abraham R T. Cell cycle checkpoint signaling through the ATM and ATR kinases [ J ]. Genes Dev, 2001 ; 15 ( 17 ) : 2177-2196.
  • 8Negrini S, Gorgoulis V G, Halazonetis T D. Genomic instability an evolving hallmark of cancer[ J]. Nat Rev Mol Cell Biol, 2010 ; 11 (3) : 220-228.
  • 9Roos W P, Kaina B. DNA damage-induced cell death by apoptosis [J]. Trends Mol Med, 2006; 12(9) : 440-450.
  • 10Shiloh Y, Kastan M B. ATM: genome stability, neuronal develop- ment, and cancer cross paths [ J ]. Adv Cancer Res, 2001 ; 83:209- 254.

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微生物学杂志
2011年 第5期

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