RNA干扰抑制人食管鳞癌细胞PLK1基因的表达

RNA Interference Inhibits PLK1 Expression in Eca-109

目的 利用RNA干扰（RNAi）技术,研究Polo样激酶l（PLK1）基因特异的siRNA对人食管鳞癌细胞Eca-109 PLKl基因表达的影响.方法将PLKI基因特异性siRNA转染入人食管鳞癌细胞Eta-109,采用RT、-PCR和Western-blot技术检测siRNA处理前后Eca-109细胞PLK1基因表达变化,并构建PI.K1基因特异性siRNA质粒表达载体.结果转染R后T-PCR和Western-blot结果均显示Eca-109细胞PLK1基因的表达明显下降,PLKI基因特异性siRNA质粒表达载体构建成功.结论 PLKl特异性siRNA对Eca-109细胞PLK1基因的表达有抑制作用.

Objective To explore the possibility of PLKI as a therapeutic target for Eca-109,RNA interference was employed to inhibit PLKI expression in Eca-109 cells. Methods siRNA oligo of PLK1 was synthesized anti transfeeled into Eta-109 cells. PLK1 mRNA expression and protein content were determined by reverse transcription polymerase chain reaction（ RT-PCR } and Western blot respectively, and construe! PLKI gene-speeifie siRNA plasmid expression vector. Results PLK1 mRNA expression and protein eontenl were significantly decreased by PLKI gene-specifie siRNA,and PLKI gene-speeifie siRNA plasmid expression vector was construe｜ed suct＇essfully. Conclusion PLKI gene-specifie siRNA can inhibil expression of PI,KI in Eea-109 cells effectively.