期刊文献+

丙氨酸消旋酶的纯化及活性测定方法 被引量:1

Purification and activity assay method of alanine racemase
下载PDF
导出
摘要 丙氨酸消旋酶广泛分布于原核生物中,在人和其他高等生物中没有它的存在。丙氨酸消旋酶用于催化L-丙氨酸和D-丙氨酸的相互转化,归为5′-磷酸吡哆醛依赖型酶,属于异构酶家族。 Alanine racemase was existed widely in Prokaryotes, but it ,wasn't existed in men and eukaryotes. Alanine racemase can be used to catalyse the change of the Optical Structure of L-- Alanine and D-- Alanine. It depended on 5'-PLP and was classified to Isomerase family.
作者 张彩凤
出处 《河北渔业》 2011年第12期38-39,共2页
关键词 丙氨酸消旋酶 纯化 活性 alanine racemase purification activity.
  • 相关文献

参考文献6

  • 1Neuhaus, F. C. , and Baddiley, J. : A continuum of anionic charge: structures and functions of D-- alanyl-- teichoic acids in grampositLve bacteria. Microbiol. MoL. Biol. Rev., 2003, 67, 686 - 723.
  • 2Strych U, Davlieva M, Longtin J P, et al. Purification and preliminarycrystallization of alanine racemase from Streptococcus pneumoniae. BMC Microbiology, 2007, 40(7) 1186/ 1471 --2180.
  • 3Ju, J. , Misono, H. , and Ohnishi, K. : Directed evolution ol bacterial alanine racemases with higher expression level. J. Biosei. Bioeng., 100 (3), 246--254 (2005a).
  • 4Hashimoto, A. , Nishikawa, T. , Oka, T. , Takahashi, K. , and Hayashi, T... Determination of free amino acid enantiomers in rat brain and serum by high- performance liquid chromatography after derivadzation with N- tert. --butyloxycarbonyl-- L -- cysteine and o -- phthaldialdehyde. J. Chromatogr., 582, 41--48 (1992).
  • 5Jiansong Ju, Haruo Misono, Kouhei Ohnishi. Directed Evolution of Baeterial Alanine Racemases with Higher Expression Level. JOURNAL OF BIOSCIENCE AND BIOENGINEERING. 2005,Vol. 100, No. 3, 246 - 254.
  • 6Young--Hee L, Yokoigawa K, Esaki N, et al. A new amino acid racemase with threonine a- epimerase activity from Pseudomonas putlda : Purification and characeterization. J Bacteriol, 1993,17S (13):4213-4217.

同被引文献6

引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部