分枝杆菌快速检测方法及其应用

Rapid method on detection of Mycobacteria and its clinical application

目的本研究旨在探讨MGITTM荧光判读器快速检测分枝杆菌方法;探讨其应用价值。方法对104例初治肺结核患者和30例健康对照痰标本进行常规涂片后,无论涂阴或涂阳,分别采用传统罗氏法(以下简称LJ),荧光判读器法(以下简称MMGIT)及MGITTM960自动培养系统(以下简称AMGIT)进行培养与检测,并对结果进行比较和评价。结果 104例结核患者中,涂片阳性55例(阳性检出率52.88%),三种培养方法检测结果分别为MMGIT阳性65例(阳性检出率62.50%),AMGIT阳性67例(阳性检出率64.42%),LJ阳性62例(阳性检出率59.62%)。30例健康对照涂片和培养均为阴性。MMGIT比LJ平均报阳时间早9.47天;其最短报阳时间比LJ快5天。与AMGIT相比,平均阳性时间晚1.56天。结论荧光判读仪检测法是一个快速敏感的分枝杆菌检测方法;与MGITTM960快速培养法功效一致,且经济实用,简单方便。值得基层结防机构临床应用。

Objective To study MGITI＇M fluorescence interpretoscope rapid method on detection of Mycobacteria and to study its clinical application value. Methods The sputum of 104 cases of active tuberculosis and 30 healthy controls were cultured for Mycobacteria using methods of LJ, MMGIT and AMGIT after smear. The results were compared with each other and were evaluated. Results In 104 tuberculosis patients,55 smears were positive （ positive rate was 52. 88% ） ; The number of the positive cultivations for LJ, AMGIT and MMGIT were 62,67 and 65, respectively; and their positive rates were 59.62%, 64.42% and 62.50%, respectively. All 30 negative controls showed 100% negative. MMGIT detected positively 9.47 days earlier than LJ and the shortest positive time was 5 days earlier than LJ. Conclusions MMGIT is a rapid and sensitive method on detection of Mycobacteria compared with LJ method, and the function of this instrument is similar to MGITTM960. It is an economic, convenient method and worth applying to more wider health populations.