特征光谱吸收法测量人血清前列腺特异性抗原浓度的研究

Research on Serum Prostate-Specific Antigen Measurement by Characteristic Spectral Absorption Method

采用免疫层析金标试纸配合特征光谱吸收法检测前列腺特异性抗原(PSA,prostate-specific anti-gen)浓度,采用合适发射光波长(520～535nm)的LED照射已用PSA标准液处理过的胶体金标记试纸,用合适接收波长(520～540nm)的光电二极管测试试纸特定区域的反射光强度,据此得出表征试纸吸光的量,再用线性拟合方法确定PSA浓度与试纸吸光度的关系。研究中采用不同浓度的PSA标准液标定了实验检测系统,发现在浓度范围为5～50ng.mL-1时,PSA的浓度与试纸的吸光度成线性关系。在该浓度范围内对不同于标定浓度的21种PSA标准液做了检测实验,检测值与真实值的相关系数为0.910。实验证明,采用特征光谱吸收法测量PSA浓度的方法是可行的。

A serum prostate-specific antigen（PSA） measure ment method was studied using immunochromatography-based colloidal gold-marked test-strips and characteristic spectral absorption method.Some LEDs in the ra nge from 520 to 535 nm irradiated the test-strips sampled by PSA,and then a p hotodiode in the range from 520 to 540 nm measured the reflex light at the spec ific area.Accordingly,the absorbency of the strip could be calculated,then th e relationship between PSA level and strip absorbency could be ascertained by li near fit method.The experimental measurement system was calibrated by different PSA standard solutions in this research.As a result,in the range between 5 t o 50 ng·mL-1,the levels of PSA have a linear relationship with the absor bency of test-strips.Some experiments were completed by 21 different PSA stand ard solutions within that range,and then the correlation coefficient of the res ults is equal to 0.910,which proves that the proposed method for serum PSA lev el measurement is feasible.