GC／MS法分析硫磺熏蒸对杭白菊挥发油成分的影响

Analysis on the Influence of Essential Oils from Hangzhou White Chrysanthemum Before and After Sulfur-Fumigated Process by GC/MS

分析杭白菊通风晾干品和硫磺熏蒸品的挥发油成分,为其质量评价提供科学依据。采用水蒸气蒸馏法提取7个杭白菊样品挥发油,用GC毛细管色谱柱进行分离,归一化法测定其相对百分含量,GC/MS法分析鉴定其化学成分。初步分离鉴定出64种成分,其挥发油组成皆以单萜类和倍半萜类化合物为主要成分,但所含化合物的具体种类有很大差异。经硫磺熏蒸后,样品中蒎烯、合成右旋龙脑、香芹酚、石竹烯氧化物等活性成分含量明显降低,而香树烯、γ-芹子烯等成分含量升高。实验结果表明,应严格控制硫磺熏蒸在杭白菊加工过程中的应用。

To analyze the constituents of the essential oils extracted from Hangzhou white chrysanthemum, one of the commercial breeds coming from the flowers of Chrysanthemummorifolium, treated by sun-dried and sulfur-fumigated process, and to provide scientific evaluation for its quality control. The essential oils of seven Hangzhou white chrysanthe- mum samples were extracted by water-steam distillation and separated by GC capillary column chromatography. The contents of the components were quantitatively determined with normalization method, and their chemical constituents were identified by GC/MS. The results showed that 64 constituents were separated and identified from the components of the essential oils of seven Hangzhou white chrysanthemum samples, and they are mostly kinds of different monoterpenoids and sesquiterpenoids compounds, The concentrations of a-pi- nene （dextro）, L-borneol, carvacrol and caryophyllene oxide after sulfur-fumigated process were much lower than that before sulfur-fumigated process. On the contrary the concentrations of （--）-alloaromadendrene and 7-selinene were higher than that before. Thus, sulfurfumigated processing of Hangzhou white chrysanthemum should be strictly controlled.