联合检测高尔基体糖蛋白-73与甲胎蛋白在原发性肝癌中的诊断意义

Significance of golgi protein 73 and alpha-fetoprotein expression in diagnosis of primary hepatic cancer

目的评价联合检测高尔基体糖蛋白-73(GP73)与甲胎蛋白(AFP)对原发性肝癌的诊断意义。方法采用酶联免疫吸附试验(ELISA)定量检测50例原发性肝癌和50例健康对照者血清GP73与AFP的表达,分别分析比较不同临床病理指标及其联合检测的意义。结果原发性肝癌组中GP73与AFP均明显高于对照组[GP73:309.56±192.33)ng/ml﹠(22.11±8.52)ng/ml;AFP:(400.31±214.49)μg/L﹠(4.55±6.87)μg/L],差异均有统计学意义(t分别=10.58、13.04,P<0.05)。GP73与AFP的联合检测敏感度最高达到85.20%,其特异性、阳性预测值和阴性预测值分别为92.30%、91.70%、86.20%。原发性肝癌患者中GP73表达与血清AFP水平、肿瘤直径、TNM分期无关(t分别=0.25、0.25、0.22,P均>0.05),与肝硬化相关(t=1.72,P<0.05)。结论 GP73高表达于PHC,可成为PHC较好的诊断标志物。联合AFP检测有助于PHC的诊断。

Objective To investigate the expression of golgi protein 73（GP73） and alpha-fetoprotein（AFP） in primary hepatic cancer（PHC） and analyze their clinical significance.Methods Fifty PHC and Fifty normal liver specimens were included in the study.The expression of GP73,and AFP in these specimens were detected by Enzyme-linked immunosorbent assay.The correlation of the expression of these proteins with clinical pathology parameters in PHC was analyzed.Results The expression levels of GP73 and AFP were significantly higher in PHC（GP73：309.56±192.33 vs.22.11±8.52;AFP：400.31±214.49 vs.4.55±6.87;t =10.58,13.04,P0.05）.The sensitivity of combined detection including GP73 and AFP for PHC was up to 85.20%,and its specificity,positive predictive value and negative predictive values were 92.30%,91.70%,86.20%.The expression of GP73 in the patients with primary hepatocellular carcinoma was not associated with serum level of AFP,TNM stage,and tumor diameter（t=0.25,0.25,0.22,P0.05）;was associated with liver cirrhosis（t=1.72,P0.05）.Conclusions GP73 is highly expressed in PHC and can be used as a diagnostic marker for PHC.Combined detection of GP73 and AFP is helpful for diagnosis of PHC.