益气化瘀中药对糖尿病大鼠皮肤溃疡组织中P物质表达的影响

Effects of Chinese herbal medicine Yiqi Huayu formula on substance Pexpression in skin ulcers of rats with diabetes mellitus

目的:探讨益气化瘀中药对糖尿病溃疡大鼠创面组织P物质表达的影响。方法:将100只清洁级雄性Sprague-Dawley大鼠随机分为正常创面组、模型组、益气组、化瘀组和益气化瘀组。制备糖尿病大鼠背部全层皮肤缺损开放性创面模型。各组分别给予生理盐水、益气中药、化瘀中药及益气化瘀中药灌胃处理,疗程为15d。用药第8及第15天,观察各组大鼠创面愈合率;用药15d后,取大鼠背部创周0.5cm以内组织,应用免疫组织化学技术检测P物质的表达水平,并计算免疫组织化学指数。结果:用药后第8及第15天,正常组创面愈合率明显高于模型组(P<0.01),各用药组创面愈合率明显高于模型组(P<0.05),且益气化瘀组明显高于各拆方组(P<0.05);模型组创面组织P物质的表达水平明显低于正常创面组(P<0.05),益气化瘀组和化瘀组P物质的表达水平明显高于模型组(P<0.05)。结论:糖尿病创面难愈合可能与P物质的低表达有关。益气化瘀中药能明显促进糖尿病溃疡大鼠的创面愈合,上调P物质的表达水平可能是其作用机制之一。

Objective： To investigate the effects of Chinese herbs for replenishing qi （Yiqi） and dissolving stasis （Huayu） on substance P expression in granulation tissue of skin ulcers in rats with diabetes mellitus. Methods： One hundred male Sprague-Dawley rats with back full-thickness skin lesion were randomly divided into five groups： ulcer control group, diabetic ulcer group, Yiqi Huayu herb group, Yiqi herb group, and Huayu herb group. Except ulcer control group, the other rats were also injected streptozotocin to induce diabetes mellitus. Rats in each group were given normal saline or herbal drugs for 15 d and the wound healing rate of the rats was observed on days 8 and 15, respectively. After treatment, the expression of substance P in granulation tissues of the rats was tested with immunohistochemical method and the immunohistochemical index was calculated.Results： On the 8th and 15th days after treatment, the wound healing rate of the ulcer control group was higher than that of the diabetic ulcer group （P〈0.01） ; the wound healing rates of all the treated groups were higher than that of the diabetic ulcer group （P〈0.05）; the wound healing rate of the Yiqi Huayu herb group was higher than those of the Yiqi herb group and the Huayu herb group （P〈0. 05）. Compared with the diabetic ulcer group, the expression of substance P of the ulcer control group was increased （P〈0.05） ; the expressions of substance P of the Yiqi Huayu herb group and the Huayu herb group were higher than that of the diabetic ulcer group （P〈0.05）. Conclusion： Yiqi Huayu herbs can promote wound healing in rats with diabetes mellitus by up-regulating the expression of substance P.