烟酸对人脐内皮细胞损伤的保护作用

Protective Effect of Niacin on Human Umbilical Vein Endothelial Cells Injury Induced by Cholesterol in Vitro

研究了烟酸对胆固醇处理的人脐静脉内皮细胞(HUVECs)损伤的保护作用。使用倒置显微镜观察细胞形态,四甲基偶氮唑蓝(MTT)比色法测定HUVECs存活率。结果表明:随着胆固醇浓度的增加,用MTT比色法测得的吸光度逐渐降低,HUVECs存活率显著降低(P<0.05)。烟酸呈剂量依赖性的显著改善胆固醇诱导所致下降的HUVECs存活率(P<0.05)。胆固醇可以诱导内皮细胞损伤,烟酸对胆固醇诱导的人脐静脉内皮细胞损伤具有保护作用。

To investigate protective effect of niacin on human umbilical vein endothelial cells （ HUVECs）, which injury treated by cholesterol. Use inverted microscope observation cells form, the growth rate of cells was de- tected by MTT （3-（4,5-dimethylthiazo 1-2-yl）-2,5-diphenylte trazolium bromide） methods. With the increase of cholesterol concentration, the value of MTY was significantly reduced （P 〈 0.05 ）. Niacin significantly improved the growth of cells with dose dependent （P 〈 0.05）. Cholesterol could induce injury for HUVECS, while niacin may prevent HUVECS from the injury.