UF-1000i尿液有形成分分析仪、干化学法和镜检法对尿液分析的比较

Comparison on UF-1000i,dry chemistry analyzer and microscopy examination for urinalysis

目的探讨Sysmex UF-1000i全自动尿液有形成分分析仪(简称UF-1000i)对尿液中红细胞(RBC)、白细胞(WBC)和管型(CAST)检测的敏感性。方法对1 054例患者的尿液分别用UF-1000i、URISys-2400全自动干化学分析仪(干化学法)及Diasys R/S2005定量分析工作站(镜检法)3种方法进行分析,并比较3种方法对RBC、WBC、CAST的检测敏感性。结果 UF-1000i检测RBC、WBC、CAST的阳性率分别为20.6%、20.3%、6.7%;干化学法检测出RBC、WBC的阳性率分别为24.5%、17.9%;镜检法检出RBC、WBC、CAST的阳性率分别为16.9%、20.5%、2.2%。3种方法RBC、CAST的检出率差异有统计学意义(P<0.01),WBC的检出率差异无统计学意义(P>0.05)。结论 UF-1000i不能完全取代尿沉渣镜检,建议3种方法联合应用以减少检验误差,提高尿液分析质量。

Objective To investigate the sensitivities of Sysmex UF-1000i urine sediment analyzer（UF-1000i） in detecting urine red blood cells（RBC）,white blood cells（WBC） and casts（CAST）.Methods Urine specimens of 1 054 patients were collected,and each sample was examined by UF-1000i,URISys-2400 automatic dry chemistry analyzer and Diasys R/S2005 microscopy.The sensitivities of the 3 urine analysis methods were compared.Results The positive rates of urine RBC,WBC and CAST were 20.6%,20.3% and 6.7% respectively by UF-1000i.The positive rates of RBC and WBC were 24.5% and 17.9% by dry chemistry analyzer respectively.The positive rates of RBC,WBC and CAST were 16.9%,20.5% and 2.2% respectively by urine sediment microscopy.The results were statistically analyzed by χ2 test.The detection rates of RBC and CAST were significantly different（P0.01）,but the detection rate of WBC did not differ significantly（P 0.05）.Conclusions Urine sediment microscopy can not be completely replaced by UF-1000i in urinalysis.The combination of these 3 methods would be optimal to improve the accuracy and precision in urinalysis.