纳米羟磷灰石-脂肪族聚酯酰胺复合材料对成骨细胞的生物学作用

Biological effects of nano-hydroxyapatite-aliphatic polyester-amide composite on the osteoblasts

目的研究纳米羟磷灰石-脂肪族聚酯酰胺(nHA-PEA)对成骨细胞的生物学作用。方法以含有nHA-PEA的达尔贝科极限必需培养(DMEM)浸提液作用于试验组细胞,DMEM作用于对照组细胞,以甲噻唑四唑氮检测nHA-PEA对成骨细胞生长的影响,流式细胞计数细胞周期的变化,酶联免疫吸附测定细胞碱性磷酸酶(AKP)活性的变化。将细胞和材料联合培养,观察细胞在复合材料上的黏附和生长情况。结果试验组细胞的相对增殖率为92%～107%且无量效关系,试验组与对照组间差异无统计学意义(P>0.05);试验组和对照组细胞的细胞周期及AKP活性表达相似,组间差异无统计学意义(P>0.05)。成骨细胞直接培养于复合材料上,显现出良好的黏附、铺展和生长行为。结论 nHA-PEA对成骨细胞的生长和功能无不良影响,具有骨细胞相容性。

Objective To evaluate the biological effects of nano-hydroxyapatite-aliphatie polyester-amide composite （nHA-PEA） on the osteoblast. Methods The Dulbecco minimum essential medium（DMEM） leaching liquor of nHA-PEA was applied to the osteoblasts of the test groups while the DMEM itself was applied to control. The methyl thiazolyl tetrazolium assay, flow cytometry and alkaline phosphatase CAKP） analysis were used to evaluate the changes in cell growth, cell cycle and ceU function. Moreover, osteoblasts were cultured on the surface of nHA-PEA composite and the attachment, growth and proliferation of osteoblast were investigated. Results The cultured osteoblasts grew well and showed nomorphological variation. Osteoblasts of different test groups demonstrated relative proliferation rate ranging from 92%-107% without dose-dependent effect（P〉0.05）. The cell cycle and AKP activity were similar in test and control groups （P〉0.05）. Good cell attachment and proliferation manner were observed on the membranes. Conclusion nHA-PEA has no negative effects on the osteoblast and its osteoblastcompatibility is proved.