原发性肝细胞癌以及近癌旁组织微血管密度CD105表达及其临床意义

Detection and clinical significance of microvascular density labeled by CD105 in hepatocellular carcinoma and adjacent non-tumorous

目的 研究肝细胞癌（HCC）及其近癌旁组织微血管密度（MVD-CD105）分布,探讨MVD-CD105在HCC、近癌旁组织的分布规律、临床意义以及CD105标染HCC组织微血管的特异性.方法 采用免疫组织化学方法检测63例HCC组织、近癌旁组织MVD-CD105,比较HCC组织和近癌旁组织MVD-CD105分布特点及其与临床参数的关系.结果 63例HCC及其近癌旁组织血管CD105阳性表达率为100％,近癌旁组织表达强度高于癌组织（χ2=9.184,P＜0.01）.HCC组织MVD-CD105为（58.37±21.45）/0.74mm2,近癌旁组织为（81.62±19.86）/0.74mm2,近癌旁组织MVD-CD105分布显著高于癌组织（t=2.438,P＜0.05）.HCC组织中的MVD-CD105阳性表达分布与肿瘤转移及TNM分期有关（P＜0.01）.近癌旁组织的MVD-CD105阳性表达分布与肿瘤TNM分期及2年存活时间有关（P＜0.05）.结论 CD105标染HCC组织MVD未体现出肿瘤微血管标染特异性.HCC组织MVD-CD105高表达、分布意味着肿瘤的局部转移和进展.CD105分子不能作为抗肝癌肿瘤血管生成靶向治疗的靶标,但肝癌MVD-CD105的检测对于后续综合治疗有指导意义.

Objective To evaluate the specificity of CD105 labeled microvessel density （MVD）and its clinical significance.Methods Formalin fixed,paraffin wax embedded specimens from 63 patients with hepatocellular carcinoma （HCC） were stained with anti-CD105 monoclonal antibody.A correlation was analyzed between the expression and distribution of MVD-CD105 in HCC/adjacent non-tumorous tissues and clinicopathological features.Results CD105 immunoreactivity was seen in all the specimens of HCC and adjacent non-tumorous tissues.Immunoreactivity intensity was higher in adjacent non-tumorous tissues than in HCC （χ2=9.184,P〈0.01）.The mean scores of M VD-CD105 were also higher in adjacent non-tumorous tissues than in HCC.The levels of expression and distribution of MVD-CD105 in HCC were associated with tumor metastasis and TNM staging （P〈0.01）.The levels of expression and distribution of MVD-CD105 in adjacent non-tumorous tissues were associated with TNM staging and 2-year survival rate （P〈0.01）.Conclusions CD105 showed no specificity in labeling MVD of HCC specimens.The higher level of expression and distribution of MVD-CD105 in HCC was associated with local tumor metastasis and progression.CD105 cannot be used as a target for anti-angiogenesis therapy for HCC.