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羽衣甘蓝ARC1的基因分离、表达及与SRK相互作用的分析 被引量：4

Isolation,Expression of ARC1 from Ornamental Kale and Interaction Analysis Between ARC1 and SRK
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摘要 以羽衣甘蓝(Brassica oleracea var.acephala)自交不亲和系(S13-bS13-b)为试验材料,利用RT-PCR的方法分离其ARC1基因cDNA序列(命名为BoARC1,GenBank登录号为EU344909)。BoARC1 cDNA序列中含有1个1 992 bp的开放读码框(ORF),编码1个含有663个氨基酸的蛋白质。羽衣甘蓝BoARC1与油菜BnARC1的氨基酸序列具有94%的一致性;Southern杂交结果显示BoARC1在基因组中只存在单一拷贝;Northern杂交结果显示BoARC1特异性地在柱头组织中表达;在酵母双杂交试验分析中,BoARC1能够与SRK3、SRK13-b和SRK910的胞内激酶域发生相互作用。 The ARC1 cDNA sequence was isolated from stigma of ornamental kal（e Brassica oleracea var.acephala）S13-bS13-b homozygotes using RT-PCR techniques,named BoARC1（GenBank accession No.EU344909）.The BoARC1 cDNA sequence had an ORF of 1 992 bp,encoding a 663-aa protein.The deduced amino acid sequence of isolated BoARC1 shared 94% identity with Brassica napus ARC1.Southern blot analysis showed that there was a single copy of BoARC1 gene in genome.Northern blot analysis showed that BoARC1 mRNA only expressed in the stigma.The yeast two-hybrid analysis indicated that the BoARC1 interacted with SRK3,SRK13-b and SRK910.
作者 蓝兴国 杨佳 赵昕 李玉花
机构地区 东北林业大学生命科学学院
出处 《园艺学报》 CAS CSCD 北大核心 2011年第12期2342-2348,共7页 Acta Horticulturae Sinica
基金 国家自然科学基金项目(30900115 31070275) 中央高校基本科研业务费专项基金项目(DL09BA08)
关键词 羽衣甘蓝 自交不亲和 ARC1 相互作用 Brassica oleracea var.acephala self-incompatibility ARC1 interaction
分类号 S681.9 [农业科学—观赏园艺]
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参考文献24

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二级参考文献74

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共引文献15

同被引文献64

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园艺学报
2011年 第12期

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