12-脂氧合酶在胰腺癌中的表达及其临床意义

Expression and clinical significance of lipoxygenase-12 in human pancreatic adenocarcinoma

目的检测胰腺癌组织和胰腺癌细胞株SW1990和PANCl的12-脂氧合酶（12-lipooxygenase，12-LOX）表达，探讨其与胰腺癌临床病理参数的关系。方法分别应用免疫组化染色、RT—PCR和蛋白质印迹法检测胰腺癌组织、癌旁正常胰腺组织及胰腺癌细胞株SW1990和PANCl中12-LOXmRNA和蛋白的表达。分析胰腺癌组织12-LOX表达与临床病理参数的相关性。结果30例胰腺癌组织12-LOX表达阴性8例，弱阳性7例，强阳性15例，总阳性率为73．3％。SW1990和PANCl细胞均表达12-LOX。阳性表达的胰腺癌组织及两株胰腺癌细胞株均表达12-LOXmRNA，而癌旁正常胰腺组织不表达12-LOXmRNA及蛋白。胰腺癌组织12-LOX强阳性表达与肿瘤TNM分期、肿瘤病理分级和淋巴结转移相关（P〈0．05），而与患者年龄、性别无关。结论12-LOX在胰腺癌中表达上调，与胰腺癌的恶性生物学行为有关。

Objectives To study the expression of lipoxygenase-12 （12-LOX） in human pancreatic adenocarcinoma and its relationship with the clinicopathological parameters. Methods Expression of 12-LOX in pancreatic carcinoma tissue, pancreatic cancer cell lines SW1990 and PANC1, and adjacent normal pancreatic tissue was detected by using immunohistochemistry, RT-PCR, and Western blot, respectively. The relationship between 12-LOX expression and clinicopathological parameters was analyzed. Results Expression of 12-LOX was negative in 8 cases, weak positive in 7 cases and strong positive in 15 cases of pancreatic carcinoma. The overall positive rate in pancreatic carcinoma was 73.3%. The expressions of 12-LOX mRNA and protein were positive in pancreatic cancer cell lines SW1990 and PANCl, and negative in adjacent normal pancreatic tissue. The strong positive expression of 12-LOX in pancreatie cancer was associated with TNM stage, pathological grade, lymph node metastasis （ P 〈 0.05 ）. Conclusions The expression of 12-LOX was up-regulated in pancreatic cancer and is correlated well to malignant behaviors of tumor.