大黄对急性坏死性胰腺炎大鼠炎性介质表达的影响

Inhibition of the expression of inflammation cytokines by rhubarb in experimental acute necrotizing pancreatitis rats

目的观察中药生大黄空肠灌注对急性坏死性胰腺炎（ANP）大鼠胰腺组织炎性介质表达的影响。方法sD大鼠33只，按完全随机法分为对照组、ANP组及生大黄治疗组，每组11只。胰胆管逆行注射3％牛磺胆酸钠溶液方法制备ANP模型，同时做空肠造瘘。生大黄组于造模后空肠灌入1g／ml生大黄煎液1ml／kg体重。造模36h后处死大鼠。取血测定淀粉酶活性；取部分胰腺组织常规病理检查；取部分胰腺组织抽提总mRNA，采用实时PCR方法测定胰腺组织IL-6、IL-8、TNF-αmRNA表达。结果造模后大鼠血淀粉酶活性明显升高，胰腺组织大片坏死、出血，大量炎细胞浸润，符合ANP改变。对照组胰腺组织IL-6、IL-8、TNF-αmRNA表达量分别为0．29±0．13、0．35±0．15、1．09±0．32：ANP组分别为2．23±0．49、2．26±0．51、5．24±0．59，均较对照组显著增加（P值均〈0．05）；生大黄组分别为0．974-0．30、1．02±0．34、2．59±0．36，均较ANP组显著减少，但仍显著高于对照组（P值均〈0．05）。结论生大黄空肠灌注治疗ANP大鼠可减少胰腺组织IL-6、IL-8、TNF-αmRNA的表达，从而减轻胰腺的病理损伤。

Objective To observe the changes of inflammation cytokines in pancreas tissues of experimental acute necrotizing pancreatitis （ANP） rats treated with rhubarb. Methods Thirty-three rats were randomly divided into sham operation group, ANP group and rhubarb treatment group with 11 rats in each group. ANP model was induced by retrograde injection of 3% sodium taurocholate into the biliopancreatic duct, and jcjunostomy was performed. Rats in rhubarb treatment group were fed with 1 g/ml rhubarb at the dose of 1 ml/kg body weight via jejunum route. Thirty-six hours later the rats were sacrificed. The serum level of amylase was measured; part of the pancreatic tissue was harvested for routine pathologic examination; total RNA was extracted from pancreatic tissue. The expression of IL-6, IL-8, and TNF-α mRNA was measured by real-time PCR. Results After ANP induction, the serum level of amylase was significantly increased, and pancreatic tissue necrosis, bleeding and inflammatory cell infiltration were observed, which was consistent with changes of ANP. The expressions of IL-6, IL-8, and TNF-α mRNA were 0.29 ± 0.13, 0.35 ±0.15, 1.09 ± 0.32 in sham operation group, while they were 2.23 ± 0.49, 2.26 ± 0.51,5.24 ± 0.59 in ANP group, which were significantly higher than those in sham operation group （ P 〈 0.05 ）. The corresponding values were 0. 97 ± 0. 30, 1.02 ± 0.34, 2.59 ± 0. 36 in rhubarb treatment group, which were significantly lower than those in ANP group, but they were still significantly higher than those in sham operation group （ P 〈 0.05 ）. Conclusions Rhubarb lavage via jejunum route can decrease the expressions of IL-6, IL-8, and TNF-α,therefore attenuate the pancreatic pathologic injuries.