MPT64检测作为结核分枝杆菌生长指标的研究

Development of a novel method for indicating growth of Mycobacterium tuberculosis by detecting MPT64

目的分析把检测MPT64作为结核分枝杆菌(Mycobacterium tuberculosis,MTB)生长指标的可行性。方法对MTB阳性液体培养物进行MPT64检测,分析其作为MTB生长指标的有效性;对不同稀释浓度(1mg/ml、0.1mg/ml、0.01mg/ml和0.001mg/ml)的MTB阳性液体培养物进行MPT64检测,分析该项检测的指示最低限;对不同放置时间(10d、30d、60d)的MTB阳性液体培养物进行MPT64检测,分析指示的稳定性。结果 20份MTB阳性液体培养物的MPT64检测均阳性,该项检测能100%有效指示MTB的生长;3份MTB阳性液体培养物1mg/ml、0.1mg/ml、0.01mg/ml稀释浓度的MPT64检测均阳性,其中2份MTB阳性液体培养物0.001mg/ml稀释浓度的MPT64检测阳性,而另1份则检测阴性,该项检测的指示最低限为0.01mg/ml;10份MTB阳性液体培养物放置30d、60d后MPT64检测均为阳性,该项检测用于指示MTB生长具有很好的稳定性。结论 MPT64检测可作为MTB生长的一种指示手段。

Objective To develop a novel method for indicating growth of MTB（Mycobacterium tuberculosis） by detecting MPT64.Methods Effectiveness,down limitation and stability of this method were analyzed respectively by detecting MPB64 of positive culture,of different-concentration dilution,of different-time preserved culture.Results All 20 positive fluid cultures of MTB were successfully conformed by detecting MPT64 and effectiveness of this method was 100%.All dilutions（1mg/ml,0.1mg/ml,0.01mg/ml and 0.001mg/ml） of 2 positive fluid culture were positive when MPT64 was detected.Three dilutions（1mg/ml,0.1mg/ml and 0.01mg/ml） were positive for 1 positive fluid culture,so down limitation was 0.01mg/ml.MPT 64 detection was still positive for all 10 positive fluid cultures after 30-day preservation and 60-day preservation.Conclusion Detection of MPT64 could be used for indicating growth of MTB.