食品中副溶血弧菌血清学分型与分子分型的研究

Analysis of pulsed-field gel electrophoresis molecular subtyping and serotyping of Vibrio parahaemolyticus in food

目的分析深圳市2007～2010年监测食品中副溶血弧菌存在的优势血清型与基因型之间的相似性,初步建立深圳市食源性副溶血弧菌脉冲场凝胶电泳分子分型监测网络。方法选择2007～2010年监测食品中分离出来的副溶血弧菌主要血清群(O1-O4)55株,基因组DNA经Sfi1酶切,通过脉冲场凝胶电泳PFGE获得图谱,利用BioNumerics分析软件对图谱进行聚类分析。结果相似性在90%以上的有15株,其中有9株相似性为100%,分别是2株O2:K28;1株O1:KUT与1株O4:K42;5株O3:K6,其余菌株谱型差异较大。结论监测食品中分离的副溶血弧菌PFGE型别多,且相互之间关系分散。PFGE分子分型监测网络的建立,有助于食源性疾病发生时的主动监测,爆发调查和传染源追踪。

Objective To analyze the relationship between genotype and serotype of Vibrio parahaemolyticus isolated from food in Shenzhen from 2007 to 2010 and develop primary molecular subtyping surveillance network of Vibrio parahaemolyticus.Methods The 55 strains of Vibrio parahaemolyticus isolated from diarrhea patients containing important serotype O1-O4,chromosomal DNAs were digested with the restriction enzyme Sfi1,and then were analyzed by pulsed-field gel electrophoresis.Results The similarity of the 15 strains was over 90% and 9 strains with 100% similarity including 2 strains O2：K28,1 strain O1：KUT and 1 strain O4：K42,5 strains O3：K6.Other strains showed prominent difference.Conclusion Various Vibrio parahaemolyticus patterns exist in food and the relationship is scattered.The development of PFGE molecular subtyping surveillance network would contribute to the surveillance,outbreak investigation and source tracking for Vibrio parahaemolyticus infection.