凋亡调控因子在早期自然流产患者绒毛滋养层细胞中的表达

Study on the correlation and the expression of apoptosis regulatory factors such as survivin and caspase-3 in the villi of the early spontaneous abortion

目的比较凋亡调控因子Survivin及caspase-3在自然流产及反复自然流产组患者绒毛中的表达,探讨两者在早期自然流产发生中的作用及相关性。方法随机抽取正常早孕组、首次自然流产组及反复自然流产组患者各30例,应用免疫组织化学SP法检测Survivin与caspase-3在绒毛滋养层细胞中的定位和表达。结果首次自然流产组及反复自然流产组患者绒毛中Survivin的表达均低于正常早孕组,而caspase-3则高于正常早孕组,差异均有显著性统计学意义(P<0.05)。两流产组间患者绒毛中这两者的表达则无统计学意义(P>0.05)。流产组患者绒毛中Survivin与caspase-3呈负相关。结论早期自然流产的发生可能与Survivin表达减少caspase-3表达增加引起滋养层细胞过度凋亡有关。

Objective To investigate the expression of survivin and caspase-3 in the villous trohocytes of normal early pregnancy women and the first spontaneous abortion and recurrent spontaneous abortion in patients.Methods The 90 first and repeated spontaneous abortion patients were randomly divided into normal early pregnancy group,first spontaneous abortion group and repeated abortion group,each consisted of 30 cases.With the method of immunohistochemical SP to examine the cell＇s position and expression of survivin and caspase-3 in the villous trophocytes.Results The expression of survivin on the villis from the two abortion groups were lower than the normal pregnancy group,but caspase-3 were higher than those of normal pregnancy group.The above differences were of statistical significance（P0.05）.The expression of these two on the villis had no statistical significance（P0.05） between the two abortion groups.Among the villis of the two abortion groups,survivin have a negative correlation with caspase-3.Conclusion The pathogenesis of early spontaneous abortion may be related to excessive apoptosis of trophoblast cells caused by reduced expression of survivin and increased expression of caspase-3,leading to villous angiogenesis obstacles.