Ki67、VEGF、nm23在乳腺癌组织中表达的相关性及临床意义

Expression correlation of Ki67,VEGF and nm23 genes in breast cancer and their clinical significance

目的探讨乳腺癌组织中Ki67、VEGF与nm23的表达及其与临床病理因素的相关性,并明确其临床意义。方法利用组织芯片技术与免疫组化方法检测120例乳腺癌及癌旁正常乳腺组织中Ki67、VEGF、nm23的表达,并结合临床病理等因素进行相关性分析。结果 Ki67、VEGF在乳腺癌组织中的表达均显著高于癌旁正常乳腺组织(P<0.05),而nm23在乳腺癌组织中的表达低于癌旁正常乳腺组织(P<0.05);Ki67的表达与乳腺癌临床分期有关(P<0.05),而与患者年龄、肿瘤大小、淋巴结转移状况无显著相关性(P>0.05);Ki67与VEGF的表达具有协同性,两者的共同表达与乳腺癌临床分期有关(P<0.05)。结论 Ki67、VEGF、nm23表达与乳腺癌的发生、发展密切相关,其中Ki67的阳性表达可以作为评价乳腺癌发展的一个重要生物学指标,联合检测VEGF、nm23对于指导乳腺癌临床分期与治疗具有重要意义。

Objective To investigate the expressions of Ki-67, vascular endothelial growth factor（VEGF） and nm23 in human breast cancer tissue and their relations to clinicopathological parameters, so identify their clinical significances. Methods The expressions of Ki-67, VEGF and nm23 was determined by immunohistochemistry and tissue micro-array（TMA） in 120 cases of breast cancer tissues and adjacent normal breast tissues. Than their correlations with clinicopathologieal factors were statistically analyzed. Results The expressions of Ki67, VEGF in the breast cancer tissues were significantly higher than the adjacent normal breast tissue（ P 〈 0.05 ）. The expression of nm23 in the breast cancer tissues was significantly lower than the adjacent normal breast tissue（ P 〈 0. 05 ）. The expression of Ki67 correlated with tumor stages （P 〈 0.05 ）, but did not relate to age, tumor size and lymph node stages. The expression of Ki67 coordinated with VEGF, and their co-expression related to tumor stages and tumor size （ P 〈 0.05 ）. Conclusions The expressions of Ki-67, VEGF and nm23 are correlated with breast cancer carcinogenesis and development. The expression of Ki67 is an objective biological marker for estimating the occurrence and progression of breast cancer. Combined detection of Ki-67, VEGF and nm23 may help to judge the clinical stages of breast cancer.