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HLA-G在结肠癌外周血中的表达及其临床意义 被引量：1

Expression of HLA-G mRNA on peripheral blood mononuclear cell in colon cancer patients and its clinical significance
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摘要 目的检测组织相容性白细胞抗原G(HLA-G)在结肠癌外周血单个核细胞(PBMC)中的表达并探讨其临床应用价值。方法采用实时荧光定量PCR相对定量检测结肠癌患者、结肠良性病变患者和健康对照外周血单个核细胞HLA-G mRNA的表达。结果外周血单个核细胞HLA-G mRNA在结肠癌、结肠良性病变和健康对照中的相对表达量95%可信区间(CI)分别为[1.15,2.68]、[0.56,1.51]和[0.66,1.41]。结肠癌患者外周血单个核细胞HLA-G mRNA的表达高于结肠良性病变(P=0.02)和健康对照(P=0.03)。结论结肠癌患者外周血单个核细胞HLA-G与结肠癌发生密切相关,在结肠癌早期诊断中具有重要作用。 Objective To evaluate the expression of HLA-G mRNA in colon cancer and its significance. Methods The expression of HLA-G mRNA on PBMC was determined by real-time PCR. Results The relative expression ratio CI of PBMC HLA-G mRNA was [ 1.15, 2.68] in colon cancer, [0.56, 1. 51 ] in colon benign lesion, [0.66, 1.41 ] in healthy individuals, respectively. The expression level of HLA-G mRNA on PBMC in colon cancer was significantly higher than that in both benign colon lesion （P = 0.02）and healthy con- trol （P = 0.03）. Conclusions HLA-G mRNA on PBMC in colon cancer was closely correlated with tumorigenesis and might play a crucial role in the colon cancer early diagnosis.
作者 徐亦君 竺明晨
机构地区 南京医科大学附属南京第一医院消化科 江苏省肿瘤医院检验科
出处 《中国肿瘤外科杂志》 CAS 2011年第6期348-350,共3页 Chinese Journal of Surgical Oncology
基金 江苏省肿瘤医院青年创新研究项目(ZQ200904)
关键词 HLA-G 外周血单个核细胞 结肠癌 PCR 结肠良性病变 HLA-G peripheral blood mononuclear cell colon cancer PCR colon benign lesion
分类号 R735.35 [医药卫生—肿瘤]
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  • 1LeMaoult J, Caumartin J, Daouya M, et al. Immune regulation by pretenders: cell-to-cell transfers of HLA-G make effector T cells act as regulatory cells. Blood, 2007, 109(5): 2040-8.
  • 2Shortman K, Naik SH. Steady-state and inflammatory dendritic-cell development. Nat Rev Immunol, 2007, 7(1): 19-30.
  • 3Gregori S. Dendritic cells in networks of immunological tolerance. Tissue Antigens, 2011, 77(2): 89-99.
  • 4Steinman RM, Hawiger D, Nussenzweig MC. Tolerogenic dendritic cells. Annu Rev Immunol, 2003, 21: 685-711.
  • 5Rutella S, Danese S, Leone G. Tolerogenic dendritic cells: cytokine modulation comes of age. Blood, 2006, 108(5): 1435 -40.
  • 6Cools N, Ponsaerts P, Van Tendeloo VF, et al. Balancing between immunity and tolerance: an interplay between dendritic cells, regulatory T cells, and effector T cells. J Leukoc Biol, 2007, 82(6): 1365-74.
  • 7Morelli AE, Thomson AW. Tolerogenic dendritic cells and the quest for transplant tolerance. Nat Rev Immunol, 2007, 7(8): 610-21.
  • 8Collison LW, Workman C J, Kuo TT, et al. The inhibitory cytokine IL-35 contributes to regulatory T-cell function. Nature, 2007, 450(7169): 566-9.
  • 9Chui CS, Li D. Role of immunolglobulin-like transcript family receptors and their ligands in suppressor T-cell- induced dendritic cell tolerization. Hum Immunol, 2009, 70(9): 686-91.
  • 10Roman A, Rodriguez M, Herraiz MA, et al. Heterogeneous expression of HLA-G1, -G2, -G5, -G6, and -G7 in myeloid and plasmacytoid dendritic cells isolated from umbilical cord blood. Hum Immunol, 2009, 70(2): 104-9.

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  • 1Gonen-Gross T,Goldman-Wohl D,Huppertz B,et al.Inhibitory NK receptor recognition of HLA-G: regula-tion by contact residues and by cell specific expres-sion at the fetal-maternal interface [ J ]. PLoS One,2010,5(1) :e8941.
  • 2Yan WH. Human leukocyte antigen-G in cancer : arethey clinically relevant [J]. Cancer Letters,2011,311(2) :123-130.
  • 3Zidi I, Ben Amor N. HLA-G regulators in cancermedicine: an outline of key requirements[ J]. TumourBiol,2011,32(6) :1071-1086.
  • 4Morandi F,Scaruffi P, Gallo F, et al. Bone marrow-infiltrating human neuroblastoma cells express highlevels of calprotectin and HLA-G proteins [ J]. PLoSOne,2012,7(1) : e29922.
  • 5Suciu-Foca N, Manavalan JS, Scotto L, et al. Mole-cular characterization of allospeciflc T suppressor andtolerogenic dendritic cells : review [ J]. Int Immuno-pharmacol, 2005,5(1) :7-11.
  • 6Rodriguez JA, Galeano L, Palacios DM,et al. AlteredHLA class I and HLA-G expression is associated withIL-10 expression in patients with cervical cancer[J].Pathobiology, 2012,79(2) :72-83.
  • 7Abediankenari S, Ghasemi M ,Kim YJ. Human leuko-cyte antigen-G expression on dendritic cells inducedby transforming growth factor-beta 1 and CD4 + Tcells proliferation[ J]. Iran Biomed J,2011,15(1-2):1-5.
  • 8Elliott RL,Jiang XP,Phillips JT,et al. Human leuko-cyte antigen G expression in breast cancer : role inimmunosuppression [ J ] . Cancer Biother Radiopharm,2011,26(2) :153-157.
  • 9Agaugue S, Carosella ED,Rouas-Freiss N. Role ofHLA-G in tumor escape through expansion ofmyeloid-derived suppressor cells and cytokinic bal-ance in favor of Th2 versus Thl/Thl7 [ J ]. Blood,2011,117(26) :7021-7031.
  • 10Yie SM,Hu Z. Human leukocyte antigen-G ( HLA-G) as a marker for diagnosis,prognosis and tumorimmune escape in human malignancies [ J ]. HistolHistopathol, 2011,26(3) :409420.

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中国肿瘤外科杂志
2011年 第6期

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