摘要
本文开展了FITC染料编码流式聚苯乙烯微球的研究。研究结果显示,制备的聚苯乙烯微球粒径为5.4μm,变异系数为3.04%。当低亮和高亮流式聚苯乙烯微球分别用0.4μL和125μL的FITC母液染色时间0.5 h时,可以得到微球群集中、荧光强度变异系数分别为5.28%4、.63%的二元流式微球阵列。FITC染色编码微球保存12个月,保留荧光强度为90%以上。
The action of FITC to flow functional beads encoding was studied in this paper.Results from this study showed that produced microbeads had the average diameter of 5.4 μm and 3.04% CV value.Flow cytometry exhibited that the high-light and low-light bead,which constructed the duplexed bead array with 5.28%,4.63% CV value,can be successfully encoded by 0.4 μL and 125 μL FITC fluorescence solution for half an hour.Ninety percent of FITC density can be kept after 12 months.
基金
山东省优秀中青年科学家科研奖励基金(BS2010YY044)
山东省高校科研项目(J08LE12)
济南市中小企业技术创新基金(2010007)
关键词
聚苯乙烯微球
编码
流式细胞仪
polystyrene beads
beads encoding
flow cytometry
