升麻提取物指纹图谱研究

Study on fingerprint chromatograms of extract of Cimicifugae Rhizoma

目的:首次研究建立升麻提取物指纹图谱。方法:采用酚酸类和三萜皂苷类指纹图谱相结合的方式,以HypersilBDS C18(4.6 mm×250 mm,5μm)为色谱柱,乙腈-0.1%磷酸水为流动相梯度洗脱,流速均为1.0 mL.min-1,柱温为30℃,测定波长分别为316,210 nm。结果:酚酸类指纹图谱以13个共有峰为评价指标,精密度和重复性中共有峰相对保留时间和相对峰面积RSD均小于3.0%,10批样品的相似度均大于0.9;三萜皂苷类指纹图谱以14个共有峰为评价指标,精密度和重复性中共有峰相对保留时间和相对峰面积RSD均小于4.0%,10批样品的相似度均大于0.9。结论:该方法全面、稳定、可靠,可以有效用于升麻提取物纯化物的质量控制,为升麻提取物与同属植物提取物的比较及药效学差异的分析提供一定的参考,也为升麻提取物的进一步开发利用奠定基础。

Objective： To establish the fingerprint chromatograms of the extract of Cimicifugae Rhizoma firstly.Method： Phenolic acids and triterpenoid saponins were analyzed by HPLC.Hypersil BDS Cl8（4.6 mm×250 mm,5 μm） column was used,the mobile phase was composed of acetonitrile-0.1% H3PO4 with gradient elution,flow rate was 1.0 mL·min-1,column temprature was 30 ℃,and the detection wavelength was set at 316 nm and 210 nm.Result： In the fingerprint of phenolic acids,thirteen feature peaks were found and the RSD of relative retention time and relative peak area were all less than 3% in the precision and repeation experiments.The similarity of ten batches of samples were all more than 0.90.In the fingerprint of triterpenoid saponins,fourteen feature peaks were found and the RSD of relative retention time and relative peak area were all less than 4% in the precision and repeation experiments.The similarity of ten batches of samples were all more than 0.90.Conclusion： This method is comprehensive,stable,reliable and can be used to evaluate the quality of the extract of Cimicifugae Rhizoma.It has provided a reference to the analysis on pharmacodynamic deferences of Cimicifuga extracts and also laid the foundation for its further development.