小檗碱对糖尿病肾病大鼠肾小球系膜细胞TGF-β_1与Ⅳ型胶原蛋白表达的影响

Effect of berberine on expression of transforming growth factor-β_1 and type Ⅳ collagen proteins in mesangial cells of diabetic rats with nephropathy

目的:探讨小檗碱对糖尿病肾病(DN)大鼠肾病变的保护作用及其机制。方法:以链脲佐菌素(STZ)复制DN大鼠模型,动物分为正常对照组、模型组、小檗碱低、中、高剂量治疗组(50,100,200 mg.kg-1.d-1)、消渴丸治疗组(XKW,0.8 g.kg-1.d-1),8周后检测空腹血糖(FBG)、肾重/体重比(KW/BW)、尿素氮(BUN)、肌酐(Cr)及尿蛋白(Upro);免疫组织化学检测肾小球系膜细胞转化生长因子β1(TGF-β1)与Ⅳ型胶原(Ⅳ-C)蛋白的表达。结果:ig给药,小檗碱可降低大鼠FBG,KW/BW,BUN,Cr及Upro水平,降低肾小球系膜细胞TGF-β1与Ⅳ-C蛋白的表达。结论:小檗碱对DN大鼠肾微循环病变具有保护作用,这种作用可能与其抑制肾组织TGF-β1与Ⅳ-C蛋白表达有关。

Objective： To explore the effect and mechanism of berberine on diabetic nephropathy in experimental rats.Method： The rat model of diabetic nephropathy was induced by injection of streptozocin（STZ）.The rats were divided into 6 groups： control group,model group,3 berberine treatment groups and Xiaoke Wan（XKW） treatment group.The fasting blood glucose（FBG）,kidney weight/body weight（KW/BW）,blood urea nitrogen（BUN）,creatinine（Cr） and urinary protein（Upro）were tested 8 weeks later.The expression of transforming growth factor-β1（TGF-β1）and type Ⅳ collagen（Ⅳ-C） proteins in renal tissue of diabetic rats with nephropathy were observed by optical micrography.Result： Berberine could reduce the levels of FBG,KW/BW,BUN,Cr,Upro and the expression of TGF-β1 and Ⅳ-C proteins in renal tissue of diabetic rats with nephropathy.Conclusion： Berberine may protect renal function and slow down the progression of diabetic nephropathy in rats by suppressing the expression of TGF-β1 and Ⅳ-C proteins in renal tissue.