眼针对脑缺血再灌注损伤大鼠脑组织细胞间黏附因子-1表达的影响

Effect of Eye-acupuncture on Cerebral Intracellular Adhesion Molecule-1 expression in Rats with Acute Cerebral Ischemia-reperfusion Injury

目的:观察眼针对脑缺血再灌注损伤大鼠脑组织细胞间黏附因子(ICAM-1)表达的影响,探讨眼针治疗脑缺血再灌注损伤的机制。方法:40只雄性SD大鼠随机分为正常组、假手术组、模型组和眼针组,每组10只。模型组和眼针组应用线栓法复制脑缺血再灌注损伤大鼠模型。眼针治疗取肝区、上焦区、下焦区、肾区进行,每日两次,每次20min。再灌注后72h进行神经功能缺损评分,采用Western blot、实时荧光定量聚合酶链反应法测定脑组织ICAM-1蛋白及mRNA表达的变化。结果:眼针组再灌注损伤72h后较模型组神经功能缺损评分有明显下降(P<0.01),眼针组大鼠脑组织ICAM-1蛋白及mRNA表达显著低于模型组(P<0.05)。结论:眼针具有明显的改善大鼠脑缺血再灌注损伤的作用,其机制可能与其下调脑组织ICAM-1表达有关。

Objective To investigate the effect of the eye-acupuncture therapy on cerebral intercellular adhesion molecule-1（ICAM-1） expression in rats with acute cerebral ischemia-reperfusion injury（CI/RI） so as to reveal its underlying mechanism.Methods Forty male SD rats were randomly and equally divided into normal,sham operation（sham）,model and eye-acupuncture groups.Acute CI/RI model was established by occlusion of the middle cerebral artery with a modified suture-blocking method.Filiform needles were inserted into the ＂Gan＂（Liver Area）,＂Shangjiao＂（Upper Energizer Area）,＂Xiajiao＂（Lower Energizer Area）,etc.of the eye-acupuncture acupoints,and retained for 20min.The treatment was conducted twice daily,7 times altogether.Seventy-two hours after Acute CI/RI,the neurological behavior was assessed by ZeaLonga neurophysical impairment scale.Cerebral ICAM-1 protein and ICAM-1 mRNA expression levels were detected by Western blot（Penumbra area of the right cerebral infarction region） and real time-polymerase chain reaction（PCR） methods,respectively.Results After modeling,the neurophysical impairment score of the model group was increased from 0 to（2.63±0.92） points on the first day and（2.63±0.74） points 72h following Acute CI/RI.Correspondingly,cerebral ICAM-1 protein and mRNA expression was up-regulated considerably in the model group than in the normal control group（P0.01）.In comparison with the model group,the neurologic impairment score,and cerebral ICAM-1 protein and mRNA expression levels of the eye-acupuncture group were down-regulated obviously（P0.01,P0.05）.No significant differences were found between the normal control and sham groups in neurologic impairment score and the expression levels of the ICAM-1 protein and ICAM-1 mRNA（P0.05）.Conclusion Eye-acupuncture therapy can improve the cerebral ischemia-reperfusion injury induced behavior changes,which may be related to its effects in down-regulating the expression of cerebral ICAM-1 protein and gene.