电针猫“足三里”穴抑制胃扩张所致的一过性下食管括约肌松弛

Electroacupuncture at "Zusanli"(ST 36) Can Inhibit Frequencies of Transient Lower Esophageal Sphincter Relaxation Induced by Gastric Distention in Cats

目的:探讨电针"足三里"穴以及"足三里"联合"内关"穴对一过性下食管括约肌松弛(TLESR)的影响及其机制。方法:健康成年猫随机分为8组:足三里组、内关组、足三里联合内关组(简称联合组)、非经非穴组、单纯胃扩张组(模型组)、生理盐水组、L-精氨酸组、他克林组,每组5只。通过逐次向胃内注气的方法建立胃扩张诱发TLESR的动物模型。各治疗组在胃扩张的同时给予电针刺激,持续60min。用低顺应性毛细管灌注测压系统记录、分析TLESR频率、共腔发生率和下食管括约肌压力(LESP)。结果:与模型组相比,足三里组、内关组和联合组均可以显著降低TLESR的频率及其合并共腔的比率(P<0.05);与电针前相比,电针"足三里"穴可以显著增加LESP(P<0.05),其它组与电针前相比差异无统计学意义(P>0.05);他克林可以逆转电针"足三里"穴对TLESR的抑制,而L-精氨酸则不能逆转。结论:电针"足三里"能够减少胃扩张引起的TLESR频率的增多和共腔的发生,此作用的发挥可能与胆碱通路有关。

Objective To observe the influence of electroacupuncture（EA） of ＂Zusanli＂（ST 36） and ST 36 ＋ ＂Neiguan＂（PC 6） on gastric distention induced transient lower esophageal sphincter relaxation（TLESR） in cats.Methods Forty cats were randomly divided into model（gastric distension） group,EA-ST 36 group,EA-PC 6 group,EA-ST 36＋PC 6 group,EA-non-acupoint group,EA-ST 36＋saline（2mL i.v.） group,EA-ST 36＋L-arginine（precusor of nitric oxide,200mg/kg i.v.） group,and EA-ST 36＋Tacrine（cholinesterase inhibitor,5.6mg/kg i.p.） group,with 5 cats being in each group.TLESR model was established by extending the stomach with an air balloon.EA（2Hz/100Hz,3-4mA） was applied to bilateral ST 36,PC 6 and ST 36＋PC 6 and non-acupoint（exterior-superior site of the hip） while gastric distension.L-Arginine was given 10min before EA,and Tacrine was given 30 min before EA.The frequency of TLESR and lower esophageal sphincter pressure（LESP） were recorded by using a perfused sleeve assembly.Results Compared with the model group,the frequency of TLESR in 60min as well as the percentage of common cavity during TLESR were significantly decreased in the EA-ST 36,EA-PC 6,EA-ST 36＋PC 6,EA-ST 36＋saline groups（P0.05）.Compared with the EA-ST 36＋saline group,the frequency of TLESR in 60 min was up-regulated significantly in the EA-ST 36＋Tacrine group（P0.01） rather than in the EA-ST 36＋L-Arginine group,suggesting a reduction of the efficacy of EA-ST 36 by cholinergic suppression（not by nitric oxide）.No significant differences were found among the EA-ST 36,EA-PC 6 and EA-ST 36＋PC 6 groups in the frequency of TLESR and the percentage of common cavity（P0.05）.In comparison with the baseline level,LESP was increased significantly only in the EA-ST 36 group（P0.05）.Conclusion EA at ST 36 can reduce both the frequency of TLESR and the percentage of common cavity during TLESR in gastric distension cats,which may be mediated by cholinergic pathway.