美洲商陆和籽粒苋对接种伯克氏菌的生理生化响应及其对富集铯的影响

The physiological and biochemical responses of P. americana Linn. and A. crenentus L. to inoculation with Burkholderia sp. and its effect to Cs accumulation

利用盆栽试验研究一株具有重金属耐性的伯克霍尔德氏菌(Burkholderia sp.D54)对美洲商陆和籽粒苋生长及对铯富集的影响.结果表明,接种菌株D54,美洲商陆和籽粒苋总干物重分别比对照处理增加19.8%～33.4%和22.9%～76.6%,美洲商陆和籽粒苋地上部铯含量分别增加4.9%～22.4%和8.1%～19.4%,根中铯含量分别增加6.8%～15.7%和1.1%～10.8%.同时,接种微生物还增加了植物对钾的吸收,提高供试植物的光合反应速率、气孔导度、蒸腾速率和水分利用率,增加铯胁迫条件下植物的抗氧化酶CAT、POD、SOD的活性,降低了MDA的含量.

To investigate the effect of bacteria Burkholderia sp. D54 on Cs uptake by P. americana Linn. and A. crenentus L. , a pot experiment with different concentrations of Cs was conducted. It was found that inoculation of Burkholderia sp. D54 on P. americana Linn. and A. creaentus L. enhanced total biomass of P. americana and A. crenentus by 19.8% - 33.4% and 22.9% -76.6% , respectively. Shoot Cs concentration of P. americana and A. crenentus increased by 4.9% - 22.4% and 8. 1%- 19.4% , while root Cs concentration increased by 6, 8% -15.7% and 1. 1% - 10. 8% , respectively. We also concluded that inoculated Burkholderia sp. D54, increased potassium uptake by two types of plants, enhanced net assimilation rate, stomatal conduction, transpiration and water use efficiency of photosynthesis of the two types of plants, increased the activity of antioxidant enzymes CAT, POD and SOD under Cs stress, and reduced plants MDA content in leaves.