血府逐瘀汤及方中桔梗、牛膝对血管内皮细胞黏附分子表达的影响

Effect of Xuefu Zhuyu Decoction and Platycodon Grandiforus Achyranthes Bidentataon in It Expression of Adhesion Molecules in Vascular Endothelial Cells of Blood Stasis Rats

目的:探讨桔梗、牛膝一升一降在血府逐瘀汤中的配伍机制,不同配伍的血府逐瘀汤对血管内皮细胞黏附分子表达的影响。方法:采用免疫组织化学方法,观察桔梗、牛膝不同配伍的血府逐瘀汤对血瘀证模型大鼠血管细胞黏附分子-1(VCAM-1)以及诱生型一氧化氮合酶(iNOS)表达的影响。结果:桔梗、牛膝不同配伍的血府逐瘀汤确能通过影响内皮细胞黏附分子表达,而改善模型大鼠血瘀状态。结论:桔梗、牛膝在血府逐瘀汤中的配伍作用,并非相互拮抗,而是相互促进。

Objective ： To study the concerted application mechanisms of Platycodon grandiforus and Achyranthes bidentataon in Xuefu Zhuyu Decoction （XZD）, the effect of XZD on adhesion molecules in vascular endothelial cells （ VECs ） of blood stasis rats. Methods ： Expression of VCAM-1, iNOS in vascular endothelial cells of blood stasis rats receiving different concerted application of Platycodon grandiforus and Achyranthes bidentataon in XZD was observed by immunohistochemical method. Results ： The expression of adhesion molecules in vascular endothelial cells of blood stasis rats could be affected by different concerted application of Platycodon grandiforus and Achyranthes bidentataon in XZD. Conclusion：The concerted application mechanisms of Platycodon grandiforus and Achyranthes bidentataon in XZD not antagonistic action but promote action.