酵母葡聚糖硫酸酯化物的结构鉴定和初步药理活性研究

Study on Chemical Properties and Pharmacological Activities of Sulfated β-(1→3)-D-glucan

对酵母β-(1→3)-D-葡聚糖降解后进行硫酸化修饰,研究其硫酸酯化物(PSC)的理化性质,初步了解其结构和药理活性。采用硫酸降解酵母β-(1→3)-D-葡聚糖后,以氯磺酸-吡啶法合成硫酸酯化物,通过紫外、红外及核磁共振等方法进行检测和鉴定;通过体外凝血实验和免疫活性测定实验对其活性进行初步研究。PSC为一相对分子质量为8000左右的多糖,硫酸化修饰后多糖的取代度为2.6。在体外,PSC具有促进脾淋巴细胞增殖的作用,同时PSC能够明显延长部分凝血酶活酶活化时间(APTT)和凝血酶时间(TT)。降解修饰酵母葡聚糖后得到的硫酸酯化产物PSC具有一定免疫增强和抗凝血活性。

In the research, a sulfated derivative ofβ- （ 1 →3 ）-D-glucan, named PSC was prepared and studied on the physicochemical properties and pharmacological activities. β- （ 1 →3 ）-D-glucan was used as a starting material. It was degraded by sulfuric acid and modified using ehlorosulfonic-pyridine method to obtain PSC. The structure of PSC was identifieated by molecular weight determination, elemental analysis, PMP precolumn derivation, IR and NMR. The basic pharmacological activities of PSC were determined by anti-coagulation test and lymphocyte transformation test. The results showed that PSC is a linear β- （ 1 →3 ）-D-glucan of approximately 8 000 u with the sulfate Ds = 2.6. In vitro, PSC showed the ability to facilitate mouse splenocyte proliferation. PSC also showed to be a potent promoter of splenocyte proliferation induced by ConA or LPS mitogens. In the anti-coagulation test, PSC significantly prolonged the activated partial thromboplastin time （APTT） and thrombin time （TT）. PSC would have anti-coagulant activity and immunological function.