一株扩展青霉生长特性及展青霉素生物合成的研究

Research on the Growth of Penicillium expansum and Patulin Biosynthesis

扩展青霉是侵染食品、水果及中药材并产生展青霉素的主要产毒菌株。文章采用正交试验法考察了碳氮源、金属元素以及不同的发酵条件对扩展青霉生长及展青霉素生物合成的影响,结果表明,有利于该菌生长及毒素合成的培养基为(%):葡萄糖1.0、淀粉2.0、(NH4)2SO41.0、MgSO4 0.5,pH值6.0,在25℃、200 r/min、10%接种量、40 mL/250 mL的摇瓶发酵条件下发酵10 d,菌丝体生物量和展青霉素含量分别达到4.725 mg/mL和126.72μg/L。

Penicillium expansum was a major patulin producing strain which infects food, fruit and＇ Chinese medicinal materials. Effect of different carbon and nitrogen sources and mental ions on the strain growth and patulin biosynthesis was investigated by Orthogonal experiment. Under the conditions of 1.0% sugar,2.0% starch, 1.0% （ NH4 ）2SO4 ,0.5%Mg^2＋ ,pH 6.0 or 25 ℃, 200 r/min,10% inoculum concentration and 40 mL medium in 250 mL flask,the strain biomass and patulin concentration were 4.725 mg/mL and 126. 72 μg/L,respectivelv.