普通野生稻DNA导入栽培稻后代的苗期耐盐性鉴定

Appraisation Salt Tolerance in Seeding Stage of Rice Progenies with Transformed Common Wild Rice(Oryza rufipogon) DNA

采用营养液水培法对普通野生稻DNA导入宁夏栽培水稻宁粳16号中获得的12份后代材料进行苗期的耐盐性鉴定。结果表明,在120 mmol/L NaCl处理下,材料D 44、D 43、D 5-24、DJ 13、DJ 24和D 5-45的平均死叶率相对较低,地上和地下部能够保持较高的含水量,说明其受盐胁迫的伤害较小;所有材料的可溶性糖含量和脯氨酸积累量都较0 mmol/L NaCl的高,其中材料D 43、D 44、D 5-24、DJ 24、DJ 25和DJ 13的可溶性糖含量提高的量较其他材料高,而脯氨酸的相对积累量较其他材料低,说明这几个材料的渗透调节能力较强,相对于其他材料较耐盐。

The salt tolerance in seeding stage of 12 rice progenies of Ningjing 16 with transformed common wild rice DNA were studied by water culture. The results showed that when the solution concentration was 120 mmol/L NaCl,the rate of average dead leaf of D 44,D 43 ,D 5-24,DJ 13 ,DJ 24 and D 5-45 were lower than other materials, the shoot and root contained more water than receptor, so the salt stress had little influence on these materials. All materials had higher soluble sugar content and proline content than 0 mmol/L NaCl. The soluble sugar content of D 43, D 44, D 5-24, DJ 24, DJ 25 and DJ 13 were higher than others, however, the proline content were lower. Therefore, these materials had strong osmoregulation ability and salt tolerance.