药用唇柱苣苔叶片分化及植株再生研究

Research on Differentiation and Plant Regeneration From in Vitro Leaf Tissues of Chirita medica D.Fang ex W.T.Wang

目的:建立濒危药用植物药用唇柱苣苔叶片分化及植株再生体系。方法:以药用唇柱苣苔(Chirita medica D.Fangex W.T.Wang)叶片为外植体,灭菌后接种到附加不同激素配比的培养基中培养,筛选各阶段合适的培养基。结果:不定芽诱导的最适培养基为MS+1.0 mg/L 6-BA+0.2 mg/L NAA,pH=6.0,诱导率为100%;最佳继代增殖培养基为MS+0.5 mg/L 6-BA+0.2 mg/L NAA,pH 6.0,增殖系数为12.6/30 d;最适合的生根培养基为1/2 MS+0.2 mg/L NAA,pH=6.0,生根率高达100%,移栽成活率为95.0%。结论:本繁殖体系可在短时间内提供大量种苗,并为种质资源的保护和可持续利用提供技术指导。

Objective： To develop differentiation and plant regeneration system from in vitro leaf tissues of Chirita medica D. Fang ex W. T. Wang, an endangered medicinal species. Methods： The leaf of Chirita medica as explants were cultured on MS supplemented with different kinds and concentrations of hormone to select optimized different phase. Results：The optimum medium for adventitious buds induction and seedling regeneration was MS ＋ 1.0 mg/L 6-BA ＋ 0.2 mg/L NAA （ pH 6.0） and the induce rate of bud regeneration was 100%. The efficient medium for high regeneration frequency was MS ＋ 0.5 mg/L 6-BA ＋ 0.2 mg/L NAA（ pH 6.0） and the proliferation coefficient was 12.6/30 d. The optimal rooting medium was 1/2 MS ＋ 0.2 mg/L NAA（pH 6.0） and the rooting rate was 100% and the rate of transplanting survival was 95.0%. Conclusions：This system could be used to develop more seedlings in short time, and to provide guidance for conserving and sustainably developing the resource.