贴壁和悬浮状态293T细胞慢病毒包装效果的比较研究

Comparison of effects on lentivirus packaging between adherent and suspended 293T cells

为提高慢病毒包装效率,比较了贴壁和悬浮293T细胞的磷酸钙法慢病毒的包装效果.结果表明,利用悬浮细胞获得的病毒滴度较贴壁细胞高近3.5倍,同时这种方法具有很好的重复性,且悬浮细胞可直接用于病毒包装,节省了24 h的细胞培养时间.

To improve the efficiency of lentivirus packaging, the influences of state of cell growth and existence on virus packaging were studied ; the effects of adherent and suspended 293T cells on packaging lentivirus were compared using calcium phosphate method. The results show that the titer of lentivirus packaged with suspension cells was nearly 3.5 folds higher than that packaged with adherent cells. Moreover, two advantages, higher repeatability and less cell culture time which was at least 24 h shorter, of lentivirus packaging using suspension cells directly were also showed in this study.