3株产β-甘露聚糖酶菌株的分离和鉴定

Isolation and identification of three strains with β-mannanase activity

从新疆极端干燥环境土壤样品中,以葡甘露聚糖(魔芋精粉)为唯一碳源,利用水解圈筛选法筛选到3株具有β-甘露聚糖酶活性的菌株.分别提取3株细菌基因组并经16S rRNA序列比对分析,确定3株菌分别属于芽孢杆菌(Bacillus sp.)、弗氏柠檬酸杆菌(Citrobacter freundii)、多粘类芽孢杆菌(Paenibacillus polymyxa).

Three strains with high β-mannanase activity were isolated from soil samples from an extreme arid environment in Xinjiang. Using konjac flour as the only carbon source, theβ-mannanase activities of these strains were identified by the size of hydrolyzed circle produced by each strain. The three strains were further identified by alignment analysis of 16S rRNA gene, which belonged to Bacillus sp. , Citrobacter freundii and Paenibacillus polymyxa, respectively.