LYVE-1及Podoplanin在小鼠Lewis肺癌模型肿瘤内表达情况的初步研究

The Preliminary Study of the Expression of LYVE-1 and Podoplanin in Mouse Model of Lewis Lung Cancer

目的检测两种淋巴管标志物LYVE-1及Podoplanin在小鼠Lewis肺癌模型肿瘤中的表达,并比较其表达特异性。方法建立小鼠Lewis肺癌肿瘤模型,检测LYVE-1、Podoplanin在肿瘤内淋巴管的表达,并利用CD34标记肿瘤内的毛细血管以便与淋巴管进行区分。结果 LYVE-1及Podoplanin染色结果并不完全重叠,Podoplanin阳性表达结果除与LYVE-1相同部分外,尚存在大量棕黄色条带状及阳性细胞结构染色,且CD34表达阴性。结论 LYVE-1较podoplanin对Lewis肺癌淋巴管的识别更为特异,背景更为清晰,与CD34联合应用可准确区分毛细淋巴管与毛细血管。

Objective To investigate the expression of LYVE-1 and Podoplanin in lewis lung cancer and compare the specific expression.Methods Establish mouse model of Lewis lung cancer,use LYVE-1,Podoplanin to detect the expression of lymphatic vessels within the tumor,and use CD34 to distinguish the lymphatic vessels and blood capillaries.Results The staining results of LYVE-1 and podoplanin are not completely identical,except the same part of LYVE-1,podoplanin was also expressed in other structures which were not the blood vessels verified by CD34.Conclusion LYVE-1 is more specific than podoplanin in identification of lymphatic vessels to the Lewis lung cancer,the background is clearer,and in combination with CD34 can accurately distinguish lymphatic vessels and blood capillary.