HLA-G基因3’端单核苷酸多态性与广西儿童哮喘的相关性研究

Association of HLA-G 3' untranslated regions polymorphism with children asthma

目的探讨HLA-G基因3’端单核苷酸多态性与广西儿童哮喘发病的关联性。方法采用基于基质辅助激光解吸电离-飞行时间质谱技术检测广西儿童支气管哮喘患者84例及正常对照儿童116例的HLA-C基因3’端单核苷酸多态性(rs1704和rs1063320)分型变化,并与HLA-DR基因型进行多元回归分析,寻找易感基因型及单体型。结果 rs1063320基因型构成比在哮喘组和对照组差异有统计学意义(P<0.05);rs1704和rs1063320位点的14 bp缺失-G单体型能明显降低儿童哮喘的发病风险;14 bp缺失-G-HLA-DRB1*15XX单体型是儿童哮喘的保护性单体型,能更明显降低哮喘的发病风险,并使哮喘病人的血清IgE水平降低(P<0.05)。结论 HLA-G基因的3’单核苷酸多态性与广西儿童哮喘的发病相关。rs1704和rs1063320位点的14 bp缺失-G单体型是儿童哮喘的保护性单体型。

Objective To investigate the correlation between single nucleotide polymorphisms in the 3＇ untranslated regions of HLA-G gene and asthma occurrence of children. Methods Totally 84 children cases with asth ma and 116 controls in Guangxi region were included into the study. The HLA-G gene single nucleotide poly-morphism （SNP） genotyping, rs1704 and rs1063320, was performed by using MassARRAY system （Sequenom） by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method （MALDI-TOF）. And multiple regression analysis was performed to explore the asthma correlation. Results There was significant difference in the distribution of the genotypes of rs1063320 between cases and controls （P 〈 0. 05 ）. The individuals with rs1704/rs1063320 14-bp deletion/G haplotype had decreased risk of asthma （odds ratio [ O^R ] = 0. 344, 95% confi- dence interval [ 95% CI] = 0. 149- 0. 799,P 〈 0. 05）, and the association with asthma of rs1704/rs1063320 14-bp deletion/G combined HLA-DRB1 ＊ 15XX haplotype was more apparent with OR value of 0. 198, 95% CI of 0. 084 0. 471 （P 〈 0. 001 ）, and with lower serum value of IgE in asthma cases （P 〈 0. 05）. Conclusion HLA-G 3＇ UTR SNPs has correlations with etiology of asthma. Having the haplotype of rs1704/rs1063320 14-bp deletion/G are protective from children asthma.