6+1 STR试剂盒和电泳凝胶EX-Q20用于DNA快速检验

Rapid DNA Identification Using 6+1 STR Kit and EX-Q20 Electrophoresis

目的建立快速个体识别STR分型方法。方法取采集于FTA上的血样200份,经打孔仪取等量血痕分别使用6+1 STR试剂盒结合新型毛细管电泳凝胶EX-Q20进行快速电泳分析和使用SinofilerTM试剂盒结合POP4胶进行电泳分析,比较两者所耗费时间和结果的差异。结果 6+1 STR试剂盒结合新型毛细管电泳凝胶能够得到全部分型结果且耗时较短。结论 6+1 STR试剂盒结合新型毛细管电泳凝胶进行STR分型,结果准确可靠,尤其适用于重特大案件中大量人员的排查、比对,能大大提高办案效率。

Objective To establish a rapid STR genotyping method for individual identification.Methods Two hundred blood samples from FTA were collected.Equal amount of blood were collected by puncher and analyzed using two methods（6＋1 STR kit in combination with EX-Q20 electrophoresis and SinofilerTM kit in combination with POP4 electrophoresis）.Consuming time and results of two methods were compared.Results 6＋1 STR kit in combination with EX-Q20 electrophoresis method can obtain all genotyping results and be shorter time.Conclusion 6＋1 STR kit in combination with EX-Q20 electrophoresis method is used to STR genotyping with accurate,reliable results and this new method is potential value in mass personnel investigation and comparison in major criminal cases.It also can raise the work efficiency.