IGF-1R基因多态性与原发性肝癌易感性的相关性研究

Correlation between IGF-1R polymorphism and genetic susceptibility to hepatocellular carcinoma

目的探讨广西肝癌高发区肝癌相关基因IGF-1R基因多态性与原发性肝癌易感性的关系。方法采用病例对照研究方法,在广西肝癌高发现场人群中选取113例原发性肝癌及113名健康对照人群。以筛选IGF-1R基因SNPrs61740868位点为遗传标志,采用聚合酶链式反应-限制性片段长度多态性方法(PCR-RFLP法)检测IGF-1R基因rs61740868多态性及其基因分布的频率。结果肝癌病例组与对照组IGF-1R基因rs61740868位点均未发生基因突变,其基因型均为CC型,两组比较差异无统计学意义(P>0.05)。结论 IGF-1R基因rs61740868位点多态性与肝癌发生无关,可能不是肝癌的危险因素之一。

Objective To explore the correlation between IGF-1R polymorphism and genetic susceptibility to hepatocellular carcinoma （HCC） in the area with high incidence of HCC in Guangxi. Methods A case-control study was conducted which in- cluded 113 HCC patients and 113 normal controls. All of the cases were from the areas with high incidence of HCC in Guangxi. The SNP locus rs 61740868 of gene IGF-1R was selected as a genetic marker,and its genotypes as well as mutation frequency were determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Results There was no mutation in SNP locus rs 61740868 of IGF-1R in both HCC cases and controls. The expressions of IGF-1R were not statistically different between the two groups （P 〉 0. 05）. Conclusion Polymorphism of IGF-1R SNP locus rs 61740868 is not associated with HCC,it may not be a risk factor for HCC.