去甲斑蝥素对NCI-H446骨转移裸鼠模型的干预作用

Inhibitory effect of norcantharidin on bone metastasis in nude mice model of NCI-H446

目的探讨去甲斑蝥素对肺癌骨转移的抑制作用。方法通过贴骨接种NCI-H446肺癌细胞制作骨转移裸鼠模型,将小鼠分为对照组、低剂量药物组、高剂量药物组等3组,评价各组药物干预骨转移的情况。观察3组小鼠肿瘤生长的变化。对各组小鼠进行X线比较及解剖、病理学观察。分别检测早期、晚期骨转移阶段各组血清PICP、PINP及BSP水平,并进行统计学分析。结果与药物组比较,对照组肿瘤生长快,肿瘤质量、体积大,实验结束时体重增长总量显著低于药物组;对照组PICP、PINP水平均明显升高;低剂量药物组PICP水平明显升高、PINP水平略有增高;高剂量药物组放射学检查显示晚期肿瘤性骨损伤组血清PICP、PINP水平升高均不明显;各组血清BSP水平均升高。结论去甲斑蝥素对肺癌骨转移具有抑制作用,血清PICP、PINP水平对小细胞肺癌骨转移具有早期诊断及疗效监测作用。

Objective To investigate the effect of norcantharidin on bone metastasis of human small cell lung cancer （SCLC）. Methods A skeletal metastasis model of SCLC was established by injecting NCI-H446 cells into the right leg of nude mice. Sixty male health BALB/C mice were divided randomly into 3 groups including control group, high-dose group and low- dose group. The effects of norcantharidin on bone metastasis and bone metabolism were investigated by X-ray,pathological and molecular biochemical detections, while the conditions of tumor growth were observed. Results Comparing to drug groups, thecontrol group were with faster tumor growth,larger tumor size, and lower total weight gain at the end of the experiment. The lev- els of serum PICP and PINP were higher in control group, but which were not statistically different from that of the drug groups. Conclusions Norcantharidin can inhibit bone metastasis of lung cancer. Serum PICP and PINP levels can be used for early di- agnosis and treatment monitoring of bone metastasis of SCLC.