VEGF-C、COX-2在细支气管肺泡癌中的表达及其意义

Expression of VEGF-C and COX-2 in bronchioalveolar carcinoma and its significance

目的探讨细支气管肺泡癌(BAC)中VEGF-C、COX-2蛋白表达及意义。方法 BAC60例为实验组,肺腺癌伴BAC20例和肺腺癌22例为对照组,采用免疫组化法分别检测VEGF-C、COX-2蛋白在3种组织中的表达并分析其临床意义。结果 VEGF-C在BAC、肺腺癌伴BAC和肺腺癌中阳性率分别为66.7%、90.0%和95.5%,各组间表达差异有统计学意义(P<0.05);VEGF-C在BAC非黏液型表达阳性率显著高于黏液型(P<0.05),伴淋巴结转移组阳性率明显高于无转移组(P<0.05),VEGF-C表达与性别、年龄、肿块部位、大小及TNM分期均无关联(P>0.05)。COX-2在BAC、肺腺癌伴BAC和肺腺癌中阳性率分别为63.3%、75.0%和77.3%,各组间表达差异无统计学意义(P>0.05);COX-2在BAC伴淋巴结转移组表达阳性率明显高于无转移组(P<0.05),肿块直径≥3cm组阳性率明显高于肿块直径<3cm组(P<0.05),COX-2表达与性别、年龄、肿块部位、病理类型及TNM分期均无关(P>0.05)。VEGF-C与COX-2表达呈正相关(r=0.269,P<0.05)。结论 VEGF-C联合COX-2检测可用于BAC侵袭、转移特性的评估及预测。

Objective To investigate the expression and clinical significance of VEGF-C and COX-2 in bronchioalveolar carci- noma （BAC）. Methods 60 cases of BAC were enrolled in experimental group;the control group contained 20 cases of pulmo- nary adenocarcinoma with BAC features and 22 cases of pulmonary adenocarcinoma. Expressions of VEGF-C and COX-2 were detected by immunohistochemical. Results Positive rates of VEGF-C in BAC, pulmonary adenocarcinoma with BAC features and pulmonary adenocarcinoma were 66.7% ,90. 0% and 95.5% respectively,the difference of which was statistically signifi- cant （P 〈 0.01 ）. The positive rates of VEGF-C were notably higher in non-mucinous BAC than that in mucinous （P 〈 0.01 ）, and in lymphonode metastasis than in that without lymphonode metastases （ P 〈 0. 05 ）. The positive rate of VEGF-C was not correlated with patients＇ gender and age, and the localization, size and TNM stage of tumor （P 〉 0. 05）. Positive rates of COX-2 in BAC,pulmonary adenocarcinoma with BAC features and pulmonary adenocarcinoma were 63.3% ,75.0% and 77.3% respectively, the difference of which was not statistically significant （P 〉 0.05）. The positive rates of COX-2 were obviously high- er in lymphonode metastasis than that without lymphonode metastases （P 〈 0. 05 ） ,in tumor with diameter 〉 3cm than that with diameter 〈 3cm （P 〈 0. 05）. The positive rate of COX-2 was not correlated with patients＇ gender and age, and the localiza- tion, size, histological type and TNM stage of tumor （ P 〉 0. 05 ）. The expression of VEGF-C was positively correlated with that of COX-2 （r = O. 269 ,P 〈 0. 05 ）. Conclusion Combined detection of VEGF-C and COX-2 could be applied in assessing and predicting the invasion and metastasis of BAC.