基于微流控芯片的姜黄素诱导MCF-7细胞凋亡的研究

Microfluidic Chip-based Apoptosis of MCF-7 Induced by Curcumin

目的探讨应用微流控芯片实现高内涵药物筛选(high content screening,HCS)的可行性。方法本文将微流控芯片技术与HCS技术相结合,通过自行设计、制作聚二甲基硅氧烷(polydimethylsiloxane,PDMS)-玻璃微流控芯片,并在芯片上实现人乳腺癌MCF-7细胞培养、脂质体转染、药物姜黄素刺激等操作,最后通过显微成像技术进行检测。结果姜黄素可以诱导MCF-7细胞凋亡,并呈浓度依赖性,同时获得了细胞在凋亡过程中一些生物信息的改变:随着姜黄素浓度的增加,细胞凋亡比例、Endo G-GFP重定位比例增大,膜通透性增加,细胞核固缩变小。结论上述微流控芯片可以为HCS技术提供良好的研究平台。

Objective To discuss the feasibility of microfluidic chip to realize high content screening （HCS）. Methods In this paper, microfluidic chip and HCS were successfully combined together. We fabricated a microfluidic chip made from polydimethylsiloxane （PDMS） and glass, then accomplished the culture of MCF-7 cell line, the transfection of Endo G-GFP, and the stimulation of curcumin on this chip, finally acquired data with a microscopic imaging system. Results Curcumin could induce the apoptosis of MCF -7, and the effect relied on the concentration. The information related to apoptosis were obtained： with the increasing of the concentration of curcumin, the percentage of apoptotic cells and the proportion of relocation of Endo G-GFP went up, the permeability of plasma membrane increased, and the nuclear size diminished. Conclusions All the results demonstrated that microfluidic chip could provide a good platform for HCS.