摘要
以西洋参成熟种子及未成熟种子为试材,在无菌操作条件下剥取种胚,将其依次接种在不同2,4-D浓度的MS培养基中,在光照和暗培养条件下分别进行培养,研究2,4-D对西洋参种胚体细胞胚发生的影响。结果表明:成熟种胚在不同浓度2,4-D中皆可发生体细胞胚,最佳浓度为0.5mg/L,光照可以促进体细胞胚发生;未成熟种胚不能形成体细胞胚。说明成熟种胚体细胞胚发生需要2,4-D诱导,未成熟种胚的表现与成熟种胚差异显著;西洋参种胚在发育控制上与人参存在明显不同。
The mature and immature Panax quinquefolium seeds were used as test materials,and the zygotic embryo were dissected out aseptically and placed on solid MS medium supplemented with different concentrations of 2,4-D in the light and dark culture conditions,effect of 2,4-D on the somatic embryogenesis of american ginseng were studied.The results showed that somatic embryogenesis could occur on the mature embryos at different concentrations of 2,4-D and the optimal concentration were 0.5 mg/L,and illumination could stimulate the process;The immature embryo could form somatic embryo.The conclusion were that 2,4-D stimulated somatic embryogenesis on the mature embryos,the responses between mature and immature embryo were apparently different;The developmental control mechanism in zygotic embryo of Panax quinquefolium was from that in Panax ginseng.
出处
《北方园艺》
CAS
北大核心
2011年第23期114-116,共3页
Northern Horticulture
基金
陕西省重点实验室科研资助项目(09JS048)
关键词
西洋参
合子胚
2
4-D
体细胞胚
Panax quinquefolium
zygotic embryo
2
4-D
somatic embryogenesis
